Document Detail

Thrombin in ischemic neuronal death.
MedLine Citation:
PMID:  16427045     Owner:  NLM     Status:  MEDLINE    
Thrombin plays a role in cerebral ischemia as rats subjected to focal cerebral ischemia were protected by the intracerebral injection of hirudin, a selective thrombin inhibitor. To separate the roles of thrombin in cell death and in coagulation, we have used an in vitro approach to test the effect of hirudin and of protease nexin-1 (PN-1), a cerebral thrombin inhibitor, on neuronal ischemia. Rat organotypic hippocampal slice cultures were subjected to oxygen (5%) and glucose (1 mmol/L) deprivation (OGD) during 30 min. Hirudin or PN-1 administered after OGD significantly prevented neuronal death in the CA1 region. After 24 h, there was a marked increase in thrombin immunoreactivity on Western blots. Thrombin therefore contributes to ischemic damage in neural tissue in vitro.
Marlise de Castro Ribeiro; Jérôme Badaut; Melanie Price; Marita Meins; Julien Bogousslavsky; Denis Monard; Lorenz Hirt
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-19
Journal Detail:
Title:  Experimental neurology     Volume:  198     ISSN:  0014-4886     ISO Abbreviation:  Exp. Neurol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370712     Medline TA:  Exp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  199-203     Citation Subset:  IM    
Neurology Service, BH 19 208, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.
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MeSH Terms
Animals, Newborn
Anoxia / complications,  drug therapy
Blotting, Western / methods
Cell Death / drug effects,  physiology
Disease Models, Animal
Dose-Response Relationship, Drug
Gene Expression Regulation / drug effects
Glucose / deficiency
Hippocampus / pathology
Hirudin Therapy / methods
Hirudins / pharmacology
Ischemia / etiology,  metabolism,  pathology*,  prevention & control
Neurons / drug effects,  metabolism
Protease Inhibitors / pharmacology
Rats, Sprague-Dawley
Thrombin / antagonists & inhibitors,  physiology*
Reg. No./Substance:
0/Hirudins; 0/Protease Inhibitors; 50-99-7/Glucose; EC

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