| Thrombin facilitates invasion of ovarian cancer along peritoneum by inducing monocyte differentiation toward tumor-associated macrophage-like cells. | |
| | |
MedLine Citation:
|
PMID: 20352429 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Peritoneal metastasis is a distinct pathologic characteristic of advanced epithelial ovarian cancer (EOC), which is the most deadly disease of the female reproductive tract. The inflammatory environment of the peritoneum in EOC contains abundant macrophages, activated thrombin, and thrombin-associated receptors. However, little is known about the mechanism by which the thrombin-macrophages interaction contributes to tumor invasion and metastasis. We investigated the phenotype and cytokine/chemokine expression of thrombin-treated peripheral blood monocytes (MOs)/macrophages, it was found that the phenotype of MOs was altered toward a TAM-like macrophage CD163(high)IL-10(high)CCL18(high)IL-8(high) after thrombin stimulation. By Matrigel invasion assay, the conditioned medium of thrombin-stimulated MOs accelerated remarkable invasion of ES-2, SKOV3, and HO-8910, which was similar to invasive cell numbers of ascites stimuli (P < 0.05) and higher than MOs medium alone (P < 0.05). IL-8 was proposed as the major chemoattractant mediating EOC invasion based on MOs mRNA and protein expression profiling. It was observed that anti IL-8 monoclonal neutralizing antibody attenuated EOC cell invasion in a concentration-dependent manner. Increased transcriptional activation of NF-kappaB p50/p65 was identified in thrombin-treated MOs. This study provided insight the role of thrombin in the regulation of EOC peritoneal invasion via "educating" MOs. |
| | |
Authors:
|
Ting Zhang; Zhengwen Ma; Ruili Wang; Ying Wang; Shujun Wang; Zhongping Cheng; Hong Xu; Xinjuan Jin; Weiping Li; Xipeng Wang |
Related Documents
:
|
18214799 - Flow cytometric evaluation of intracellular cytokine synthesis in peripheral mononuclea... 6460939 - In vitro cytochemical and cytophysiological study of in vivo activated mouse peritoneal... 6500699 - Effect of thioglycolate on phagocytic and microbicidal activities of peritoneal macroph... 17485289 - Apoptosis and endometriosis. 20531289 - Ppar-γ agonist rosiglitazone protects peritoneal membrane from dialysis fluid-induced ... 9686629 - Factors increasing severity of peritonitis in long-term peritoneal dialysis patients. 19853019 - P53 mediates nitric oxide-induced apoptosis in murine neural progenitor cells. 17698589 - Memory cd8+ t cells mediate antibacterial immunity via ccl3 activation of tnf/roi+ phag... 12032269 - Up-regulation of il-18 and il-12 in the ileum of neonatal rats with necrotizing enteroc... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-30 |
Journal Detail:
|
Title: Cancer immunology, immunotherapy : CII Volume: 59 ISSN: 1432-0851 ISO Abbreviation: Cancer Immunol. Immunother. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-04-28 Completed Date: 2010-06-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8605732 Medline TA: Cancer Immunol Immunother Country: Germany |
Other Details:
|
Languages: eng Pagination: 1097-108 Citation Subset: IM |
Affiliation:
|
Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200001, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibodies, Monoclonal
/
pharmacology Cell Differentiation / drug effects* Cell Line Cell Line, Tumor Cell Movement / drug effects Cells, Cultured Chemokines / genetics, metabolism Cytokines / genetics, metabolism Female Flow Cytometry Gene Expression / drug effects Hemostatics / pharmacology Humans Interleukin-8 / genetics, immunology, metabolism Macrophages / drug effects*, metabolism, pathology Monocytes / drug effects*, metabolism, pathology NF-kappa B / metabolism Neoplasm Invasiveness Ovarian Neoplasms / genetics, metabolism, pathology Peritoneum / pathology Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / drug effects Thrombin / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Chemokines; 0/Cytokines; 0/Hemostatics; 0/Interleukin-8; 0/NF-kappa B; EC 3.4.21.5/Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Th17 cells: positive or negative role in tumor?
Next Document: Complications of definitive open reduction and internal fixation of pilon fractures of the distal ti...