| Three-step purification of a fragment of the large immunophilin FKBP52. | |
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MedLine Citation:
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PMID: 10681043 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PPIases catalyze the interconversion of cis and trans isomers of peptidyl-prolyl (Xaa-Pro) bonds in peptide and protein substrates. The PPIase family comprises three subfamilies, two of which interact with immunosuppressant drugs and are therefore termed immunophilins. One subgroup of the immunophilins are the FK506 binding proteins (FKBPs). FKBPs of a relative molecular mass higher than 40 000 also display chaperone activity and are part of the multichaperone complex that Hsp90 forms with substrate proteins. Their function in this chaperone complex is still enigmatic. To further characterize the function of FKBP52 we want to analyze constructs of FKBP52-fragments. Here we describe a fast and effective three-step purification procedure for a fragment of FKBP52 with a relative molecular mass of 48000, termed FKBP52-123, consisting of affinity chromatography, anion-exchange column and gel-permeation chromatography. A yield of 1 mg pure protein per gram of cells was achieved. |
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Authors:
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F Pirkl |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 737 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-03-01 Completed Date: 2000-03-01 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 71-6 Citation Subset: IM |
Affiliation:
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Institut für Organische Chemie und Biochemie. Technische Universität München, Garching, Germany. franziska.pirkl@ch.tum.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Immunophilins / chemistry* Peptide Fragments / isolation & purification* Recombinant Proteins / isolation & purification Tacrolimus Binding Proteins |
| Chemical | |
Reg. No./Substance:
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0/Peptide Fragments; 0/Recombinant Proteins; EC 5.2.1.-/Tacrolimus Binding Proteins; EC 5.2.1.8/Immunophilins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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