Document Detail


Three spectroscopic techniques evaluated as a tool to study the effects of iodination of monoclonal antibodies, exemplified by rituximab.
MedLine Citation:
PMID:  21723685     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Radioiodinated monoclonal antibodies have been used for radioimmunotherapeutic and radiodiagnostic purposes. Radioiodination of monoclonal antibodies may lead to deterioration of the immunoreactivity of the monoclonal antibody. Methods for the determination of the immunoreactivity, however, do not provide information about any structural changes of the radioconjugate which may influence the binding properties of the protein to the target antigen. Within this study we demonstrated the potential role of three alternative spectroscopic analytical techniques to characterize the structural changes emerging after iodination of rituximab. We conclude that techniques as liquid chromatography coupled to mass spectrometry, fluorescence emission spectrophotometry, and circular dichroism can provide valuable information about structural changes of a radiolabeled compound, e.g. during pharmaceutical development and for quality control.
Authors:
Ly Tran; Joke Baars; Carola Damen; Jos Beijnen; Alwin Huitema
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-20
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  -     ISSN:  1873-264X     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Slotervaart Hospital, Department of Pharmacy & Pharmacology, P.O. Box 90440, 1006 BK Amsterdam, The Netherlands.
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