Document Detail


Three red cell sodium transport systems in hypertensive and normotensive Utah adults.
MedLine Citation:
PMID:  6327514     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sodium-lithium countertransport (SLC), sodium-potassium cotransport (CoT), and ouabain binding to sodium-potassium adenosine triphosphatase (Na, K-ATPase) sites were measured on fresh erythrocytes from hypertensive and normotensive Utah subjects with and without a first-degree relative with hypertension. SLC was measured as Li+ efflux into NaCl and MgCl2 media from Li+-loaded cells (5-7 mM). CoT was measured by monitoring Na+ and K+ efflux from cells loaded to 20-30 mM Na+ and 20-30 mMK+. Ouabain binding was determined for fresh cells using 3H-ouabain. Subjects were selected from pedigrees that showed a prevalence of hypertension. SLC was significantly elevated in 26.5% of the hypertensive subjects (p less than 0.001) as well as in 12.8% of the normotensives with a hypertensive first-degree relative (p less than 0.05). Although elevated SLC and decreased CoT have previously been associated with hypertension, no hypertensive subject in this study exhibited both abnormalities. All subjects with elevated SLC had normal CoT. A positive correlation between SLC and CoT was observed. Few hypertensive subjects (11.8%) had decreased CoT. In the majority of subjects studied, both SLC and CoT were normal: hypertensives 61.8%, normotensives with a hypertensive first-degree relative 61.7%, and other normotensives 58.7%. The number of ouabain-binding sites was not significantly altered among hypertensives, or their relatives, even though there was a positive correlation between SLC and the number of ouabain-binding sites.
Authors:
J B Smith; K O Ash; S C Hunt; W M Hentschel; W Sprowell; M M Dadone; R R Williams
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hypertension     Volume:  6     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:    1984 Mar-Apr
Date Detail:
Created Date:  1984-07-09     Completed Date:  1984-07-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  159-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Binding Sites
Biological Transport
Erythrocytes / metabolism*
Female
Humans
Hypertension / blood*,  genetics
Lithium / blood
Male
Middle Aged
Ouabain / blood
Pedigree
Potassium / blood
Sodium / blood*
Sodium-Potassium-Exchanging ATPase / blood
Utah
Grant Support
ID/Acronym/Agency:
HL 24855-04/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
630-60-4/Ouabain; 7439-93-2/Lithium; 7440-09-7/Potassium; 7440-23-5/Sodium; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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