| Three members of the Arabidopsis glycosyltransferase family 8 are xylan glucuronosyltransferases. | |
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MedLine Citation:
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PMID: 22706449 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Xylan is a major component of the plant cell wall and the most abundant noncellulosic component in the secondary cell walls that constitute the largest part of plant biomass. Dicot glucuronoxylan consists of a linear backbone of β(1,4)-linked xylose residues substituted with α(1,2)-linked glucuronic acid (GlcA). Although several genes have been implicated in xylan synthesis through mutant analyses, the biochemical mechanisms responsible for synthesizing xylan are largely unknown. Here, we show evidence for biochemical activity of GUX1 (for GlcA substitution of xylan 1), a member of Glycosyltransferase Family 8 in Arabidopsis (Arabidopsis thaliana) that is responsible for adding the glucuronosyl substitutions onto the xylan backbone. GUX1 has characteristics typical of Golgi-localized glycosyltransferases and a K(m) for UDP-GlcA of 165 μm. GUX1 strongly favors xylohexaose as an acceptor over shorter xylooligosaccharides, and with xylohexaose as an acceptor, GlcA is almost exclusively added to the fifth xylose residue from the nonreducing end. We also show that several related proteins, GUX2 to GUX5 and Plant Glycogenin-like Starch Initiation Protein6, are Golgi localized and that only two of these proteins, GUX2 and GUX4, have activity as xylan α-glucuronosyltransferases. |
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Authors:
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Emilie A Rennie; Sara Fasmer Hansen; Edward E K Baidoo; Masood Z Hadi; Jay D Keasling; Henrik Vibe Scheller |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-06-15 |
Journal Detail:
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Title: Plant physiology Volume: 159 ISSN: 1532-2548 ISO Abbreviation: Plant Physiol. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-08-03 Completed Date: 2012-12-10 Revised Date: 2013-04-01 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1408-17 Citation Subset: IM |
Affiliation:
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Feedstocks Division, Joint BioEnergy Institute, Emeryville, California 94608, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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enzymology* Arabidopsis Proteins / genetics, isolation & purification, metabolism* Glucuronosyltransferase / metabolism* Glycosyltransferases / metabolism* Microsomes / enzymology Models, Molecular Phylogeny Protein Transport Recombinant Proteins / isolation & purification, metabolism Subcellular Fractions / enzymology Tobacco / metabolism Xylans / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Recombinant Proteins; 0/Xylans; EC 2.4.-/GUX1 protein, Arabidopsis; EC 2.4.-/GUX2 protein, Arabidopsis; EC 2.4.-/Glycosyltransferases; EC 2.4.1.17/Glucuronosyltransferase |
| Comments/Corrections | |
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