| Three immunoidentical cytochromes P-450 from liver microsomes of phenobarbital-treated rats. | |
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MedLine Citation:
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PMID: 6414466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three forms of cytochrome P-450 were purified to homogeneity from liver microsomes of Wistar-strain rats treated with phenobarbital. They had minimum mol.wts. of 52 000, 53 000 and 54 000 as determined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and are designated as P-450(L), P-450(M) and P-450(H) respectively. They were shown to be immunoidentical by Ouchterlony double-diffusion analysis. Several criteria, such as isoelectric points, substrate specificities and sensitivities to tryptic digestion, however, indicated that these cytochromes are distinct isoenzymes of cytochrome P-450. Whereas P-450(L) was highly active on various substrates, P-450(H) had generally low catalytic activities, except on aminopyrine. The cytochromes purified by immunoaffinity chromatography using anti-P-450(L) showed a marked variation in their distribution depending on the strain and colony of rat. Limited tryptic digestion of P-450(H) gave one tryptic peptide showing the same mobility as P-450(L) by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and their primary structures were very similar. The result suggests a possibility that such limited proteolysis is involved in the post-translational modification of the cytochrome or its destruction. |
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Authors:
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H Sakai; Y Hino; S Minakami |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 215 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1983 Oct |
Date Detail:
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Created Date: 1983-11-23 Completed Date: 1983-11-23 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 83-89 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cytochrome P-450 Enzyme System / immunology*, isolation & purification Electrophoresis, Polyacrylamide Gel Immunodiffusion Isoenzymes / immunology*, isolation & purification Male Microsomes, Liver / drug effects, enzymology* Phenobarbital / pharmacology* Rats Rats, Inbred Strains Species Specificity Spectrophotometry Substrate Specificity Trypsin |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 50-06-6/Phenobarbital; 9035-51-2/Cytochrome P-450 Enzyme System; EC 3.4.21.4/Trypsin |
| Comments/Corrections | |
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