Document Detail

Three-dimensional volumetric measurement of red blood cell motion using digital holographic microscopy.
MedLine Citation:
PMID:  19488109     Owner:  NLM     Status:  MEDLINE    
Measurement of blood flow with high spatial and temporal resolutions in a three-dimensional (3D) volume is a challenge in biomedical research fields. In this study, digital holographic microscopy is used to measure the 3D motion of human red blood cells (RBCs) in a microscale volume. The cinematographic holography technique, which uses a high-speed camera, enabled the continuous tracking of individual RBCs in a microtube flow. Several autofocus functions that quantify the sharpness of reconstructed RBC images are evaluated to locate the accurate depthwise position of RBCs. In this study, the squared Laplacian function yields the smallest depth of focus and locates the depthwise positions of RBCs with a root mean square error of 2.3 microm. By applying this method, we demonstrate the measurement of four-dimensional (space and time) trajectories as well as 3D velocity profiles of RBCs. The measurement uncertainties of the present method are also discussed.
Yong-Seok Choi; Sang-Joon Lee
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Publication Detail:
Type:  Evaluation Studies; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied optics     Volume:  48     ISSN:  1539-4522     ISO Abbreviation:  Appl Opt     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-02     Completed Date:  2009-08-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0247660     Medline TA:  Appl Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2983-90     Citation Subset:  IM    
Center for Bio-fluid and Biomimetics Research, Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
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MeSH Terms
Blood Flow Velocity
Erythrocytes / physiology*
Holography / methods
Imaging, Three-Dimensional
Microscopy, Video / methods
Optical Devices
Optical Phenomena

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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