Document Detail

Three-dimensional structure of TspO by electron cryomicroscopy of helical crystals.
MedLine Citation:
PMID:  20541505     Owner:  NLM     Status:  MEDLINE    
The 18 kDa TSPO protein is a polytopic mitochondrial outer membrane protein involved in a wide range of physiological functions and pathologies, including neurodegeneration and cancer. The pharmacology of TSPO has been extensively studied, but little is known about its biochemistry, oligomeric state, and structure. We have expressed, purified, and characterized a homologous protein, TspO from Rhodobacter sphaeroides, and reconstituted it as helical crystals. Using electron cryomicroscopy and single-particle helical reconstruction, we have determined a three-dimensional structure of TspO at 10 A resolution. The structure suggests that monomeric TspO comprises five transmembrane alpha helices that form a homodimer, which is consistent with the dimeric state observed in detergent solution. Furthermore, the arrangement of transmembrane domains of individual TspO subunits indicates a possibility of two substrate translocation pathways per dimer. The structure provides the first insight into the molecular architecture of TSPO/PBR protein family that will serve as a framework for future studies.
Vladimir M Korkhov; Carsten Sachse; Judith M Short; Christopher G Tate
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Structure (London, England : 1993)     Volume:  18     ISSN:  1878-4186     ISO Abbreviation:  Structure     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-14     Completed Date:  2010-09-20     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  101087697     Medline TA:  Structure     Country:  United States    
Other Details:
Languages:  eng     Pagination:  677-87     Citation Subset:  IM    
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MeSH Terms
Cryoelectron Microscopy / methods*
Membrane Proteins / chemistry
Mitochondrial Membranes / metabolism*
Protein Structure, Secondary
Rhodobacter sphaeroides / metabolism
Grant Support
MC_U105184308//Medical Research Council; MC_U105184322//Medical Research Council; MC_U105197215//Medical Research Council; //Medical Research Council
Reg. No./Substance:
0/Detergents; 0/Membrane Proteins

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