Document Detail


Three-dimensional structure of TspO by electron cryomicroscopy of helical crystals.
MedLine Citation:
PMID:  20541505     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The 18 kDa TSPO protein is a polytopic mitochondrial outer membrane protein involved in a wide range of physiological functions and pathologies, including neurodegeneration and cancer. The pharmacology of TSPO has been extensively studied, but little is known about its biochemistry, oligomeric state, and structure. We have expressed, purified, and characterized a homologous protein, TspO from Rhodobacter sphaeroides, and reconstituted it as helical crystals. Using electron cryomicroscopy and single-particle helical reconstruction, we have determined a three-dimensional structure of TspO at 10 A resolution. The structure suggests that monomeric TspO comprises five transmembrane alpha helices that form a homodimer, which is consistent with the dimeric state observed in detergent solution. Furthermore, the arrangement of transmembrane domains of individual TspO subunits indicates a possibility of two substrate translocation pathways per dimer. The structure provides the first insight into the molecular architecture of TSPO/PBR protein family that will serve as a framework for future studies.
Authors:
Vladimir M Korkhov; Carsten Sachse; Judith M Short; Christopher G Tate
Related Documents :
9831525 - Chemical basis for alkali cation selectivity in potassium-channel proteins.
19073605 - Crystal structure of epiphyas postvittana takeout 1 with bound ubiquinone supports a ro...
16925555 - Crystal structure of the apo-perr-zn protein from bacillus subtilis.
16211515 - Rapid isolation of single-chain antibodies for structural genomics.
19496755 - Proteomics identification and annotation of proteins of a cell line of bombyx mori, bmn...
3801025 - Temperature dependence of length of elastin and its polypentapeptide.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Structure (London, England : 1993)     Volume:  18     ISSN:  1878-4186     ISO Abbreviation:  Structure     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-14     Completed Date:  2010-09-20     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  101087697     Medline TA:  Structure     Country:  United States    
Other Details:
Languages:  eng     Pagination:  677-87     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cryoelectron Microscopy / methods*
Detergents
Membrane Proteins / chemistry
Mitochondrial Membranes / metabolism*
Protein Structure, Secondary
Rhodobacter sphaeroides / metabolism
Grant Support
ID/Acronym/Agency:
MC_U105184308//Medical Research Council; MC_U105184322//Medical Research Council; MC_U105197215//Medical Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/Detergents; 0/Membrane Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  An approach for de novo structure determination of dynamic molecular assemblies by electron cryomicr...
Next Document:  Metal ion roles and the movement of hydrogen during reaction catalyzed by D-xylose isomerase: a join...