Document Detail


Three-dimensional second-harmonic generation imaging of fibrillar collagen in biological tissues.
MedLine Citation:
PMID:  22752952     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Multiphoton-induced second-harmonic generation (SHG) has developed into a very powerful approach for in depth visualization of some biological structures with high specificity. In this unit, we describe the basic principles of three-dimensional SHG microscopy. In addition, we illustrate how SHG imaging can be utilized to assess collagen fibrils in biological tissues. Some technical considerations are also addressed. Curr. Protoc. Cytom. 61:6.33.1-6.33.11. © 2012 by John Wiley & Sons, Inc.
Authors:
Jiansong Xie; John Ferbas; Gloria Juan
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]     Volume:  Chapter 6     ISSN:  1934-9300     ISO Abbreviation:  Curr Protoc Cytom     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100899351     Medline TA:  Curr Protoc Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit6.33     Citation Subset:  IM    
Affiliation:
Department of Medical Sciences, Amgen, Thousand Oaks, California.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Total Internal Reflection Fluorescence (TIRF) Microscopy Illuminator for Improved Imaging of Cell Su...
Next Document:  Ex vivo imaging of excised tissue using vital dyes and confocal microscopy.