Document Detail

Three-dimensional reconstructions of the primary palate region in normal human embryos.
MedLine Citation:
PMID:  8116884     Owner:  NLM     Status:  MEDLINE    
Our knowledge of the precise spatial relationships of human primary palate morphogenesis remains poorly defined. This is due to intrinsic difficulties that exist in the study of the subject matter and a lack of adequate methodologies. We present a novel new method to allow precise three-dimensional (3-D) visualization of developing embryonic structures in previously sectioned embryos. In our study we focus on human primary palate development. Five normal human embryos from the Carnegie collection were used. 3-D reconstructions appear similar to scanning electron micrographs (SEM); however, unlike in SEM studies, the original specimen has been previously sectioned histologically. 3-D reconstruction from serial sections involved 1) histological preparation of specimen, 2) projection onto digitizing board, 3) digitization, 4) automated reassembly, and 5) relay to interactive optical disc recorder. Detailed observations of each reconstruction were then made. Data generated in this manner may also be used in the near future for quantitative morphometrics. Thus, 3-D reconstruction techniques presented in this paper generated precise spatial information on the development of the human primary palate.
F P Rudé; L Anderson; D Conley; R F Gasser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Anatomical record     Volume:  238     ISSN:  0003-276X     ISO Abbreviation:  Anat. Rec.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-03-25     Completed Date:  1994-03-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370540     Medline TA:  Anat Rec     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  108-13     Citation Subset:  IM    
Department of Orthodontics, University of North Carolina, Chapel Hill 27599.
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MeSH Terms
Cleft Lip / embryology
Cleft Palate / embryology
Epithelium / embryology
Gestational Age
Histological Techniques
Image Processing, Computer-Assisted / methods*
Palate / embryology*
Grant Support

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