Document Detail

Three-dimensional reconstruction and quantitative analysis of rat lung type II cells: a computer-based study.
MedLine Citation:
PMID:  4061335     Owner:  NLM     Status:  MEDLINE    
The three-dimensional structure of alveolar epithelial type II cells was imaged using a computer-based system designed for reconstruction and quantitative analysis of serially sectioned specimens. Six type II cells were reconstructed from serial ultrathin sections of lungs from two Sprague Dawley male rats and the results were compared to standard morphometric estimates of type II cell composition from five other Sprague Dawley male rats. A minor portion of the type II cell surface was in contact with the alveolar airspace while most of the cell surface was embedded in the alveolar septal interstitium. The type II cells contained multiple Golgi regions located close to the nucleus. Mitochondria formed a few branching filamentous networks extending throughout the cell. The reconstructed cells appeared to represent a homogeneous population having fractional volumes of intracellular organelles very similar to those found by morphometric techniques. The spatial distribution of secretory organelle volume suggests that the organization of this cell type reflects an ordered progression of secretory particle maturation which is consistent with earlier hypotheses of lamellar body assembly.
S L Young; E K Fram; B L Craig
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of anatomy     Volume:  174     ISSN:  0002-9106     ISO Abbreviation:  Am. J. Anat.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-12-18     Completed Date:  1985-12-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376312     Medline TA:  Am J Anat     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-14     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / ultrastructure
Cytoplasm / ultrastructure
Epithelium / ultrastructure
Golgi Apparatus / ultrastructure
Lung / ultrastructure*
Microscopy, Electron
Mitochondria / ultrastructure
Organoids / ultrastructure
Pulmonary Alveoli / ultrastructure
Rats, Inbred Strains
Tissue Preservation
Grant Support
R01-32188//PHS HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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