Document Detail


Three-dimensional polymer scaffolds for high throughput cell-based assay systems.
MedLine Citation:
PMID:  18405966     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many whole cell-based assays in use today rely on flat, two-dimensional (2D) glass or plastic substrates that may not produce results characteristic of in vivo conditions. In this study, a three-dimensional (3D) cell-based assay platform was established by integrating 3D synthetic polymer scaffolds with standard cell culture dishes and multi-well plates. This technology can be used to feasibly modify any traditional 2D cell-based assay vessels for 3D cell-based assay with currently used high throughput screening (HTS) systems. We examined neural stem (NS) cells' growth profile, morphology, cell-matrix interaction, gene expression and voltage gated calcium channel (VGCC) functionality of this novel 3D assay platform. Our results showed that unlike the NS cells cultured on traditional 2D planar surfaces, cells in 3D scaffolds are more physiologically relevant with respect to in vivo characteristics exhibited by in-vivo surrogates such as neural spheres. This new biomimetic cell-based assay platform may provide a broadly applicable 3D cell-based system for use in drug discovery programs and other research fields.
Authors:
Ke Cheng; Yinzhi Lai; William S Kisaalita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-04-10
Journal Detail:
Title:  Biomaterials     Volume:  29     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-04-21     Completed Date:  2008-09-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  2802-12     Citation Subset:  IM    
Affiliation:
Cellular Bioengineering Laboratory, Faculty of Engineering and Department of Biological & Agricultural Engineering, University of Georgia, Athens, GA 30602, USA.
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MeSH Terms
Descriptor/Qualifier:
Biocompatible Materials / chemistry*
Biological Assay / methods
Blotting, Western
Cell Culture Techniques / instrumentation,  methods
Cell Line
Humans
Microscopy, Electron, Scanning
Neurons / cytology,  metabolism,  ultrastructure
Oligonucleotide Array Sequence Analysis
Polymers / chemistry*
Porosity
Stem Cells / cytology*,  metabolism,  ultrastructure
Tissue Scaffolds
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Polymers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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