| Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation. | |
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MedLine Citation:
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PMID: 20603661 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell-encapsulated hydrogels with complex three-dimensional (3D) structures were fabricated from photopolymerizable poly(ethylene glycol) diacrylate (PEGDA) using modified 'top-down' and 'bottoms-up' versions of a commercially available stereolithography apparatus (SLA). Swelling and mechanical properties were measured for PEGDA hydrogels with molecular weights (M(w)) ranging from 700 to 10 000 Daltons (Da). Long-term viability of encapsulated NIH/3T3 cells was quantitatively evaluated using an MTS assay and shown to improve over 14 days by increasing the M(w) of the hydrogels. Addition of adhesive RGDS peptide sequences resulted in increased cell viability, proliferation, and spreading compared to pristine PEG hydrogels of the same M(w). Spatial 3D layer-by-layer cell patterning was successfully demonstrated, and the feasibility of depositing multiple cell types and material compositions into distinct layers was established. |
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Authors:
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Vincent Chan; Pinar Zorlutuna; Jae Hyun Jeong; Hyunjoon Kong; Rashid Bashir |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-07-05 |
Journal Detail:
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Title: Lab on a chip Volume: 10 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-27 Completed Date: 2010-09-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 2062-70 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Cell Culture Techniques / methods* Cell Survival / physiology Hydrogel / chemistry* Mice Microscopy, Fluorescence Molecular Weight NIH 3T3 Cells Oligopeptides / chemistry Photochemistry / instrumentation, methods* Polyethylene Glycols / chemistry Tissue Scaffolds |
| Chemical | |
Reg. No./Substance:
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0/Oligopeptides; 0/Polyethylene Glycols; 0/poly(ethylene glycol)diacrylate; 25852-47-5/Hydrogel; 91037-65-9/arginyl-glycyl-aspartyl-serine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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