Document Detail

Three-dimensional partial zona dissection for preimplantation genetic diagnosis and assisted hatching.
MedLine Citation:
PMID:  9988403     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To develop a new approach to partial zona dissection of oocytes and embryos for facilitating preimplantation genetic sampling and assisted hatching. DESIGN: Controlled clinical study. SETTING: Preimplantation genetic diagnosis and IVF program, Reproductive Genetics Institute/IVF Illinois, Chicago, Illinois. PATIENT(S): Three hundred forty patients undergoing IVF in whom preimplantation genetic diagnosis or assisted hatching was required. INTERVENTION(S): Three-dimensional partial zona dissection and conventional partial zona dissection were performed, with the use of a simple microneedle, on embryos before preimplantation genetic sampling or on day 3 of embryo development before ET. MAIN OUTCOME MEASURE(S): Pregnancy rate, implantation rate, and ease of preimplantation genetic sampling. RESULT(S): A pregnancy rate of 42% and an embryo implantation rate of 17.6% were obtained in the group in which three-dimensional partial zona dissection was performed, compared with a pregnancy rate of 33% and an implantation rate of 14.7% in the control group, which underwent conventional partial zona dissection. Preimplantation genetic sampling can be performed without distortion of the blastomere configuration in the embryo. CONCLUSION(S): Three-dimensional partial zona dissection is a safe and simple mechanical means of creating an opening sufficient for the atraumatic removal of material from oocytes and embryos for preimplantation genetic diagnosis and assisted hatching.
J Cieslak; V Ivakhnenko; G Wolf; S Sheleg; Y Verlinsky
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Fertility and sterility     Volume:  71     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-02-26     Completed Date:  1999-02-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  308-13     Citation Subset:  IM    
Reproductive Genetics Institute, Illinois Masonic Medical Center, Chicago 60657, USA.
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MeSH Terms
Embryo Implantation
Fertilization in Vitro*
Genetic Testing / methods*
Pregnancy Rate
Preimplantation Diagnosis / methods*
Zona Pellucida*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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