Document Detail


Three dimensional neuronal cell cultures more accurately model voltage gated calcium channel functionality in freshly dissected nerve tissue.
MedLine Citation:
PMID:  23049767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been demonstrated that neuronal cells cultured on traditional flat surfaces may exhibit exaggerated voltage gated calcium channel (VGCC) functionality. To gain a better understanding of this phenomenon, primary neuronal cells harvested from mice superior cervical ganglion (SCG) were cultured on two dimensional (2D) flat surfaces and in three dimensional (3D) synthetic poly-L-lactic acid (PLLA) and polystyrene (PS) polymer scaffolds. These 2D- and 3D-cultured cells were compared to cells in freshly dissected SCG tissues, with respect to intracellular calcium increase in response to high K(+) depolarization. The calcium increases were identical for 3D-cultured and freshly dissected, but significantly higher for 2D-cultured cells. This finding established the physiological relevance of 3D-cultured cells. To shed light on the mechanism behind the exaggerated 2D-cultured cells' functionality, transcriptase expression and related membrane protein distributions (caveolin-1) were obtained. Our results support the view that exaggerated VGCC functionality from 2D cultured SCG cells is possibly due to differences in membrane architecture, characterized by uniquely organized caveolar lipid rafts. The practical implication of use of 3D-cultured cells in preclinical drug discovery studies is that such platforms would be more effective in eliminating false positive hits and as such improve the overall yield from screening campaigns.
Authors:
Yinzhi Lai; Ke Cheng; William Kisaalita
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-09-25
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-10     Completed Date:  2013-04-22     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e45074     Citation Subset:  IM    
Affiliation:
Cellular Bioengineering Laboratory, College of Engineering, University of Georgia, Athens, Georgia, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism*
Calcium Channels / metabolism*
Caveolae / physiology
Caveolin 1 / genetics,  metabolism
Cell Culture Techniques*
Coated Materials, Biocompatible / chemistry
DNA-Directed RNA Polymerases / genetics,  metabolism
Gene Expression
Ion Channel Gating / physiology
Lactic Acid / chemistry
Mice
Molecular Imaging
Polymers / chemistry
Polystyrenes / chemistry
Superior Cervical Ganglion / cytology,  metabolism*
Tissue Scaffolds
Chemical
Reg. No./Substance:
0/Calcium Channels; 0/Caveolin 1; 0/Coated Materials, Biocompatible; 0/Polymers; 0/Polystyrenes; 26100-51-6/poly(lactic acid); 50-21-5/Lactic Acid; 7440-70-2/Calcium; EC 2.7.7.6/DNA-Directed RNA Polymerases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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