Document Detail


Three-dimensional myocardial scarring along myofibers after coronary ischemia-reperfusion revealed by computerized images of histological assays.
MedLine Citation:
PMID:  25347856     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Adverse left ventricular (LV) remodeling after acute myocardial infarction is characterized by LV dilatation and development of a fibrotic scar, and is a critical factor for the prognosis of subsequent development of heart failure. Although myofiber organization is recognized as being important for preserving physiological cardiac function and structure, the anatomical features of injured myofibers during LV remodeling have not been fully defined. In a mouse model of ischemia-reperfusion (I/R) injury induced by left anterior descending coronary artery ligation, our previous histological assays demonstrated that broad fibrotic scarring extended from the initial infarct zone to the remote zone, and was clearly demarcated along midcircumferential myofibers. Additionally, no fibrosis was observed in longitudinal myofibers in the subendocardium and subepicardium. However, a histological analysis of tissue sections does not adequately indicate myofiber injury distribution throughout the entire heart. To address this, we investigated patterns of scar formation along myofibers using three-dimensional (3D) images obtained from multiple tissue sections from mouse hearts subjected to I/R injury. The fibrotic scar area observed in the 3D images was consistent with the distribution of the midcircumferential myofibers. At the apex, the scar formation tracked along the myofibers in an incomplete C-shaped ring that converged to a triangular shape toward the end. Our findings suggest that myocyte injury after transient coronary ligation extends along myofibers, rather than following the path of coronary arteries penetrating the myocardium. The injury pattern observed along myofibers after I/R injury could be used to predict prognoses for patients with myocardial infarction.
Authors:
Monica Y Katz; Yoichiro Kusakari; Hiroko Aoyagi; Jason K Higa; Chun-Yang Xiao; Ahmed Z Abdelkarim; Karra Marh; Toshinori Aoyagi; Anthony Rosenzweig; Scott Lozanoff; Takashi Matsui
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Publication Detail:
Type:  Journal Article     Date:  2014-07-16
Journal Detail:
Title:  Physiological reports     Volume:  2     ISSN:  2051-817X     ISO Abbreviation:  Physiol Rep     Publication Date:  2014  
Date Detail:
Created Date:  2014-10-28     Completed Date:  2014-10-28     Revised Date:  2014-11-13    
Medline Journal Info:
Nlm Unique ID:  101607800     Medline TA:  Physiol Rep     Country:  United States    
Other Details:
Languages:  eng     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.
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