| Three-dimensional mass spectral imaging of HeLa-M cells - sample preparation, data interpretation and visualisation. | |
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MedLine Citation:
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PMID: 21416529 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Time-of-flight secondary ion mass spectrometry (ToFSIMS) is being applied increasingly to the study of biological systems where the chemical specificity of mass spectrometry and the high lateral resolution imaging capabilities can be exploited. Here we report a comparison of two cell sample preparation methods and demonstrate how they influence the outcome of the ToFSIMS analysis for three-dimensional (3D) imaging of biological cells using our novel buncher-ToF instrument (J105 3D Chemical Imager) equipped with a C(60) primary ion beam. Cells were analysed fixed and freeze-dried and non-fixed, frozen-hydrated. It is concluded that maintaining the cells in a non-fixed frozen-hydrated state during the analysis helps reduce chemical redistribution, producing cleaner spectra and improved chemical contrast in both 2D and 3D imaging. Insights into data interpretation are included and we present methods for 3D reconstruction of the data using multivariate analysis techniques. Copyright © 2011 John Wiley & Sons, Ltd. |
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Authors:
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John S Fletcher; Sadia Rabbani; Alex Henderson; Nicholas P Lockyer; John C Vickerman |
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Publication Detail:
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Type: Journal Article Date: 2011-03-14 |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 25 ISSN: 1097-0231 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 925-32 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
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Manchester Interdisciplinary Biocentre, School of Chemical Engineering and Analytical Science, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. john.fletcher@manchester.ac.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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