Document Detail


Three-dimensional mammalian cell growth on nonwoven polyester fabric disks.
MedLine Citation:
PMID:  7522468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Small disks of nonwoven polyester fabric (NWPF), similar to Fibra-Cel carriers, were surface treated with strong acid and used as a high surface area support matrix for in vitro culture of anchorage dependent MRC-5 cells. The disks can be autoclaved, and require inocula concentrations of only 2-5% of the final cell densities, which have reached 10(8) cells/mL of disk bed volume. Scanning electron microscopy photographs shown herein reveal that cells grow in a multilayered fashion between the randomly arrayed fibers of the nonwoven fabric, emulating in vivo growth.
Authors:
S A Petti; A C Lages; M V Sussman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology progress     Volume:  10     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    1994 Sep-Oct
Date Detail:
Created Date:  1994-11-17     Completed Date:  1994-11-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  548-50     Citation Subset:  B    
Affiliation:
Department of Chemical Engineering, Tufts University, Medford, Massachusetts 02155.
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion
Cell Division*
Cell Line
Culture Media
Fibroblasts / cytology*
Hematoxylin
Humans
Microscopy, Electron, Scanning
Polyesters*
Staining and Labeling
Chemical
Reg. No./Substance:
0/Culture Media; 0/Polyesters; 517-28-2/Hematoxylin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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