Document Detail


Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy.
MedLine Citation:
PMID:  17177275     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The organization of collagen during fibrotic processes is poorly characterized because of the lack of appropriate methodologies. Here we show that multimodal multiphoton microscopy provides novel insights into lung fibrosis. We characterize normal and fibrotic pulmonary tissue in the bleomycin model, and show that second-harmonic generation by fibrillar collagen reveals the micrometer-scale three-dimensional spatial distribution of the fibrosis. We find that combined two-photon excited fluorescence and second-harmonic imaging of unstained lung tissue allows separating the inflammatory and fibrotic steps in this pathology, underlining characteristic features of fibroblastic foci in human Idiopathic Pulmonary Fibrosis samples. Finally, we propose phenomenological scores of lung fibrosis and we show that they unambiguously sort out control and treated mice, with a better sensitivity and reproducibility in the subpleural region. These results should be readily generalized to other organs, as an accurate method to assess extracellular matrix remodeling during fibrosis.
Authors:
Ana-Maria Pena; Aurélie Fabre; Delphine Débarre; Joëlle Marchal-Somme; Bruno Crestani; Jean-Louis Martin; Emmanuel Beaurepaire; Marie-Claire Schanne-Klein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microscopy research and technique     Volume:  70     ISSN:  1059-910X     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-05     Completed Date:  2007-04-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  162-70     Citation Subset:  IM    
Affiliation:
Laboratory for Optics and Biosciences, Ecole Polytechnique, 91128 Palaiseau, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bleomycin
Collagen / ultrastructure*
Extracellular Matrix / ultrastructure*
Humans
Lung / pathology*
Macrophages, Alveolar / pathology
Male
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence, Multiphoton / methods*
Pleura / pathology
Pulmonary Fibrosis / chemically induced,  pathology*
Chemical
Reg. No./Substance:
11056-06-7/Bleomycin; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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