| Three-dimensional imaging of solvent-cleared organs using 3DISCO. | |
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MedLine Citation:
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PMID: 23060243 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The examination of tissue histology by light microscopy is a fundamental tool for investigating the structure and function of organs under normal and disease states. Many current techniques for tissue sectioning, imaging and analysis are time-consuming, and they present major limitations for 3D tissue reconstruction. The introduction of methods to achieve the optical clearing and subsequent light-sheet laser scanning of entire transparent organs without sectioning represents a major advance in the field. We recently developed a highly reproducible and versatile clearing procedure called 3D imaging of solvent-cleared organs, or 3DISCO, which is applicable to diverse tissues including brain, spinal cord, immune organs and tumors. Here we describe a detailed protocol for performing 3DISCO and present its application to various microscopy techniques, including example results from various mouse tissues. The tissue clearing takes as little as 3 h, and imaging can be completed in ∼45 min. 3DISCO is a powerful technique that offers 3D histological views of tissues in a fraction of the time and labor required to complete standard histology studies. |
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Authors:
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Ali Ertürk; Klaus Becker; Nina Jährling; Christoph P Mauch; Caroline D Hojer; Jackson G Egen; Farida Hellal; Frank Bradke; Morgan Sheng; Hans-Ulrich Dodt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-10-11 |
Journal Detail:
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Title: Nature protocols Volume: 7 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-12 Completed Date: 2013-03-19 Revised Date: 2013-03-21 |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 1983-95 Citation Subset: IM |
Affiliation:
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Department of Neuroscience, Genentech, South San Francisco, California, USA. erturk.ali@gene.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / anatomy & histology*, blood supply Furans / chemistry* Green Fluorescent Proteins / analysis, chemistry Half-Life Imaging, Three-Dimensional / methods* Mice Mice, Transgenic Microscopy, Confocal Microscopy, Fluorescence / methods* Phenyl Ethers / chemistry* Solvents / chemistry* Spinal Cord / anatomy & histology*, blood supply |
| Chemical | |
Reg. No./Substance:
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0/Furans; 0/Phenyl Ethers; 0/Solvents; 147336-22-9/Green Fluorescent Proteins; 2O6CNO27RJ/dibenzyl ether; 3N8FZZ6PY4/tetrahydrofuran |
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