Document Detail


Three-dimensional imaging of solvent-cleared organs using 3DISCO.
MedLine Citation:
PMID:  23060243     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The examination of tissue histology by light microscopy is a fundamental tool for investigating the structure and function of organs under normal and disease states. Many current techniques for tissue sectioning, imaging and analysis are time-consuming, and they present major limitations for 3D tissue reconstruction. The introduction of methods to achieve the optical clearing and subsequent light-sheet laser scanning of entire transparent organs without sectioning represents a major advance in the field. We recently developed a highly reproducible and versatile clearing procedure called 3D imaging of solvent-cleared organs, or 3DISCO, which is applicable to diverse tissues including brain, spinal cord, immune organs and tumors. Here we describe a detailed protocol for performing 3DISCO and present its application to various microscopy techniques, including example results from various mouse tissues. The tissue clearing takes as little as 3 h, and imaging can be completed in ∼45 min. 3DISCO is a powerful technique that offers 3D histological views of tissues in a fraction of the time and labor required to complete standard histology studies.
Authors:
Ali Ertürk; Klaus Becker; Nina Jährling; Christoph P Mauch; Caroline D Hojer; Jackson G Egen; Farida Hellal; Frank Bradke; Morgan Sheng; Hans-Ulrich Dodt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-11
Journal Detail:
Title:  Nature protocols     Volume:  7     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-12     Completed Date:  2013-03-19     Revised Date:  2013-03-21    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1983-95     Citation Subset:  IM    
Affiliation:
Department of Neuroscience, Genentech, South San Francisco, California, USA. erturk.ali@gene.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / anatomy & histology*,  blood supply
Furans / chemistry*
Green Fluorescent Proteins / analysis,  chemistry
Half-Life
Imaging, Three-Dimensional / methods*
Mice
Mice, Transgenic
Microscopy, Confocal
Microscopy, Fluorescence / methods*
Phenyl Ethers / chemistry*
Solvents / chemistry*
Spinal Cord / anatomy & histology*,  blood supply
Chemical
Reg. No./Substance:
0/Furans; 0/Phenyl Ethers; 0/Solvents; 147336-22-9/Green Fluorescent Proteins; 2O6CNO27RJ/dibenzyl ether; 3N8FZZ6PY4/tetrahydrofuran

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