Document Detail


Three-dimensional growth matrix for human embryonic stem cell-derived neuronal cells.
MedLine Citation:
PMID:  22611014     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The future of tissue engineering applications for neuronal cells will require a supportive 3D matrix. This particular matrix should be soft, elastic and supportive for cell growth. In this study, we characterized the suitability of a 3D synthetic hydrogel matrix, PuraMatrix™, as a growth platform for human embryonic stem cell (hESC)-derived neural cells. The viability of the cells grown on top of, inside and under the hydrogel was monitored. The maturation and electrical activity of the neuronal networks inside the hydrogel were further characterized. We showed that cells stayed viable on the top of the PuraMatrix™ surface and growth of the neural cells and neural processes was good. Further, hESC-derived neurons, astrocytes and oligodendrocytes all grew, matured and migrated when cultured inside the hydrogel. Importantly, neuronal cells were able to form electrically active connections that were verified using microelectrode array. Thus, PuraMatrix is a good supportive growth matrix for human neural cells and may serve as a matrix for neuronal scaffolds in neural tissue engineering. Copyright © 2012 John Wiley & Sons, Ltd.
Authors:
Laura Ylä-Outinen; Tiina Joki; Mari Varjola; Heli Skottman; Susanna Narkilahti
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-18
Journal Detail:
Title:  Journal of tissue engineering and regenerative medicine     Volume:  -     ISSN:  1932-7005     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101308490     Medline TA:  J Tissue Eng Regen Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Affiliation:
NeuroGroup, Institute of Biomedical Technology, University of Tampere, Tampere, Finland; BioMediTech, Tampere, Finland; The Science Center of Pirkanmaa Hospital District, Tampere University Hospital, Tampere, Finland.
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