Document Detail


Three-dimensional finite element modeling of pericellular matrix and cell mechanics in the nucleus pulposus of the intervertebral disk based on in situ morphology.
MedLine Citation:
PMID:  20376522     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nucleus pulposus (NP) cells of the intervertebral disk (IVD) have unique morphological characteristics and biologic responses to mechanical stimuli that may regulate maintenance and health of the IVD. NP cells reside as single cell, paired or multiple cells in a contiguous pericellular matrix (PCM), whose structure and properties may significantly influence cell and extracellular matrix mechanics. In this study, a computational model was developed to predict the stress-strain, fluid pressure and flow fields for cells and their surrounding PCM in the NP using three-dimensional (3D) finite element models based on the in situ morphology of cell-PCM regions of the mature rat NP, measured using confocal microscopy. Three-dimensional geometries of the extracellular matrix and representative cell-matrix units were used to construct 3D finite element models of the structures as isotropic and biphasic materials. In response to compressive strain of the extracellular matrix, NP cells and PCM regions were predicted to experience volumetric strains that were 1.9-3.7 and 1.4-2.1 times greater than the extracellular matrix, respectively. Volumetric and deviatoric strain concentrations were generally found at the cell/PCM interface, while von Mises stress concentrations were associated with the PCM/extracellular matrix interface. Cell-matrix units containing greater cell numbers were associated with higher peak cell strains and lower rates of fluid pressurization upon loading. These studies provide new model predictions for micromechanics of NP cells that can contribute to an understanding of mechanotransduction in the IVD and its changes with aging and degeneration.
Authors:
Li Cao; Farshid Guilak; Lori A Setton
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-04-08
Journal Detail:
Title:  Biomechanics and modeling in mechanobiology     Volume:  10     ISSN:  1617-7940     ISO Abbreviation:  Biomech Model Mechanobiol     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-26     Completed Date:  2011-04-27     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  101135325     Medline TA:  Biomech Model Mechanobiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanical Phenomena
Biomedical Engineering
Computer Simulation
Elastic Modulus
Extracellular Matrix / physiology
Finite Element Analysis
Humans
Imaging, Three-Dimensional
Intervertebral Disc / anatomy & histology,  physiology*
Microscopy, Confocal
Models, Anatomic
Models, Biological*
Pressure
Rats
Grant Support
ID/Acronym/Agency:
AG015768/AG/NIA NIH HHS; AR047442/AR/NIAMS NIH HHS; AR048182/AR/NIAMS NIH HHS; AR048852/AR/NIAMS NIH HHS; AR050245/AR/NIAMS NIH HHS; P01 AR050245/AR/NIAMS NIH HHS; P01 AR050245-02/AR/NIAMS NIH HHS; P01 AR050245-03/AR/NIAMS NIH HHS; P01 AR050245-04/AR/NIAMS NIH HHS; P01 AR050245-05/AR/NIAMS NIH HHS; P01 AR050245-06/AR/NIAMS NIH HHS; R01 AG015768/AG/NIA NIH HHS; R01 AG015768-07/AG/NIA NIH HHS; R01 AG015768-08/AG/NIA NIH HHS; R01 AG015768-09/AG/NIA NIH HHS; R01 AG015768-10/AG/NIA NIH HHS; R01 AG015768-11/AG/NIA NIH HHS; R01 AR047442/AR/NIAMS NIH HHS; R01 AR047442-04/AR/NIAMS NIH HHS; R01 AR047442-05/AR/NIAMS NIH HHS; R01 AR047442-06/AR/NIAMS NIH HHS; R01 AR047442-07/AR/NIAMS NIH HHS; R01 AR047442-08/AR/NIAMS NIH HHS; R01 AR048182/AR/NIAMS NIH HHS; R01 AR048182-02/AR/NIAMS NIH HHS; R01 AR048182-03/AR/NIAMS NIH HHS; R01 AR048182-04A1/AR/NIAMS NIH HHS; R01 AR048852/AR/NIAMS NIH HHS; R01 AR048852-01A2/AR/NIAMS NIH HHS; R01 AR048852-02/AR/NIAMS NIH HHS
Comments/Corrections

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