| Three-dimensional computer reconstruction of large tissue volumes based on composing series of high-resolution confocal images by GlueMRC and LinkMRC software. | |
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MedLine Citation:
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PMID: 14601147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Computer-based visualization of large tissue volumes with high resolution based on composing series of high-resolution confocal images is presented. GlueMRC and LinkMRC programs are introduced, implementing composition of overlapping series of optical sections captured by a confocal microscope, registration and subsequent composition of successive confocal stacks. Both programs are using an interactive approach in combination with automatic algorithms for image registration. Further, the method for obtaining surface renderings of microscopical structure under study is described. For this purpose, structure contours visible in the sections are interactively digitized using a Colon plug-in module running in Ellipse environment. Then the coordinates of the contours are processed by special modules in the graphic programming environment IRIS Explorer and the structure surface is rendered. The method is shown on the 3-D reconstruction of the capillary bed of human placental villi and chick embryonic gut and its vascular bed. |
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Authors:
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Petr Karen; Marie Jirkovská; Zoltán Tomori; Erna Demjénová; Jirí Janácek; Lucie Kubínová |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microscopy research and technique Volume: 62 ISSN: 1059-910X ISO Abbreviation: Microsc. Res. Tech. Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-11-05 Completed Date: 2003-12-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9203012 Medline TA: Microsc Res Tech Country: United States |
Other Details:
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Languages: eng Pagination: 415-22 Citation Subset: IM |
Copyright Information:
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Copyright 2003 Wiley-Liss, Inc. |
Affiliation:
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Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic. karen@biomed.cas.cz |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Capillaries / ultrastructure* Chick Embryo Chorionic Villi / blood supply, ultrastructure Digestive System / blood supply, embryology, ultrastructure Humans Image Processing, Computer-Assisted / methods* Imaging, Three-Dimensional / methods* Microscopy, Confocal / methods Software* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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