Document Detail


Three-dimensional computer reconstruction of large tissue volumes based on composing series of high-resolution confocal images by GlueMRC and LinkMRC software.
MedLine Citation:
PMID:  14601147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Computer-based visualization of large tissue volumes with high resolution based on composing series of high-resolution confocal images is presented. GlueMRC and LinkMRC programs are introduced, implementing composition of overlapping series of optical sections captured by a confocal microscope, registration and subsequent composition of successive confocal stacks. Both programs are using an interactive approach in combination with automatic algorithms for image registration. Further, the method for obtaining surface renderings of microscopical structure under study is described. For this purpose, structure contours visible in the sections are interactively digitized using a Colon plug-in module running in Ellipse environment. Then the coordinates of the contours are processed by special modules in the graphic programming environment IRIS Explorer and the structure surface is rendered. The method is shown on the 3-D reconstruction of the capillary bed of human placental villi and chick embryonic gut and its vascular bed.
Authors:
Petr Karen; Marie Jirkovská; Zoltán Tomori; Erna Demjénová; Jirí Janácek; Lucie Kubínová
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microscopy research and technique     Volume:  62     ISSN:  1059-910X     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-11-05     Completed Date:  2003-12-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  415-22     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Affiliation:
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic. karen@biomed.cas.cz
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MeSH Terms
Descriptor/Qualifier:
Animals
Capillaries / ultrastructure*
Chick Embryo
Chorionic Villi / blood supply,  ultrastructure
Digestive System / blood supply,  embryology,  ultrastructure
Humans
Image Processing, Computer-Assisted / methods*
Imaging, Three-Dimensional / methods*
Microscopy, Confocal / methods
Software*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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