| Three-dimensional capillary geometry in gut tissue. | |
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MedLine Citation:
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PMID: 12845569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Accurate characterization of capillary geometry is of the utmost importance for physiological tissue studies such as oxygen transport. We show that 3D microscopy can be used to measure tissue capillary geometry both in normal and disease states. We imaged fluorescently labeled gut mucosa capillary beds of three control rats and three rats 4 hours after i.p. injection of 9 mg/kg endotoxin. We used serial optical sectioning microscopy coupled with deconvolution to reconstruct 3D capillary geometry. Theoretical point spread functions accounting for depth into the specimen resulted in better reconstructions than experimentally measured point spread functions. We next derived the distribution of the shortest distances to the nearest capillary from all extravascular tissue voxels. In normal rats the shortest-distance distributions were remarkably constant despite widely varying capillary geometry. Furthermore, the mean of the shortest-distance distributions increased significantly for endotoxemic rats (4.8+/-0.4 microm) compared to controls (4.3+/-0.3 microm, P<0.05). Hence, serial sectioning microscopy provides an accurate venue for measuring physiologically relevant 3D capillary structure. |
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Authors:
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Sorel Bosan; Thoma Kareco; Dietrich Ruehlmann; Kevin Y M Chen; Keith R Walley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microscopy research and technique Volume: 61 ISSN: 1059-910X ISO Abbreviation: Microsc. Res. Tech. Publication Date: 2003 Aug |
Date Detail:
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Created Date: 2003-07-07 Completed Date: 2003-09-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9203012 Medline TA: Microsc Res Tech Country: United States |
Other Details:
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Languages: eng Pagination: 428-37 Citation Subset: IM |
Copyright Information:
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Copyright 2003 Wiley-Liss, Inc. |
Affiliation:
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UBC McDonald Research Laboratories, St. Paul's Hospital, Vancouver, BC, Canada V6Z 1Y6. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Capillaries / anatomy & histology*, drug effects Endotoxins / pharmacology Image Processing, Computer-Assisted Imaging, Three-Dimensional / methods* Intestines / blood supply*, drug effects Male Microscopy, Confocal Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Endotoxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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