Document Detail

Three-dimensional bioprinting of rat embryonic neural cells.
MedLine Citation:
PMID:  19369905     Owner:  NLM     Status:  MEDLINE    
We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites.
Wonhye Lee; Jason Pinckney; Vivian Lee; Jong-Hwan Lee; Krisztina Fischer; Samuel Polio; Je-Kyun Park; Seung-Schik Yoo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  20     ISSN:  1473-558X     ISO Abbreviation:  Neuroreport     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-13     Completed Date:  2009-08-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  798-803     Citation Subset:  IM    
Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
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MeSH Terms
Artificial Organs
Biocompatible Materials
Cell Aggregation / drug effects,  physiology
Cell Culture Techniques
Cell Proliferation / drug effects
Cell Survival / physiology
Cells, Cultured
Collagen / pharmacology
Culture Media / chemistry,  pharmacology
Extracellular Matrix / chemistry
Hydrogels / pharmacology
Neurons / cytology,  drug effects,  physiology*
Organ Culture Techniques
Printing / methods*
Spheroids, Cellular / cytology,  physiology
Stem Cells / cytology,  drug effects,  physiology*
Tissue Engineering / methods*
Tissue Scaffolds / trends*
Reg. No./Substance:
0/Biocompatible Materials; 0/Culture Media; 0/Hydrogels; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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