Document Detail

Three-dimensional automated nanoparticle tracking using Mie scattering in an optical microscope.
MedLine Citation:
PMID:  21375530     Owner:  NLM     Status:  Publisher    
The forward scattering of light in a conventional inverted optical microscope by nanoparticles ranging in diameter from 10 to 50 nm has been used to automatically and quantitatively identify and track their location in three-dimensions with a temporal resolution of 200 ms. The standard deviation of the location of nominally stationary 50-nm-diameter nanoparticles was found to be about 50 nm along the light path and about 5 nm in the plane perpendicular to the light path. The method is based on oscillating the microscope objective along the light path using a piezo actuator and acquiring images with the condenser aperture closed to a minimum to enhance the effects of diffraction. Data processing in the time and spatial domains allowed the location of particles to be obtained automatically so that the technique has potential applications both in the processing of nanoparticles and in their use in a variety of fields including nanobiotechnology, pharmaceuticals and food processing where a simple optical microscope maybe preferred for a variety of reasons.
J-M Gineste; P Macko; E A Patterson; M P Whelan
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-7
Journal Detail:
Title:  Journal of microscopy     Volume:  -     ISSN:  1365-2818     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 The Authors Journal of Microscopy © 2011 Royal Microscopical Society.
Systems Toxicology Unit, Institute for Health and Consumer Protection, European Commission DG Joint Research Centre, Italy Department of Chemical Engineering and Materials Science, Michigan State University, Michigan, U.S.A. Department of Mechanical Engineering, Michigan State University, East Lansing, MI 48824, U.S.A.
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