| The three-dimensional structure of [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough: a hydrogenase without a bridging ligand in the active site in its oxidised, "as-isolated" state. | |
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MedLine Citation:
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PMID: 20026074 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hydrogen is a good energy vector, and its production from renewable sources is a requirement for its widespread use. [NiFeSe] hydrogenases (Hases) are attractive candidates for the biological production of hydrogen because they are capable of high production rates even in the presence of moderate amounts of O(2), lessening the requirements for anaerobic conditions. The three-dimensional structure of the [NiFeSe] Hase from Desulfovibrio vulgaris Hildenborough has been determined in its oxidised "as-isolated" form at 2.04-A resolution. Remarkably, this is the first structure of an oxidised Hase of the [NiFe] family that does not contain an oxide bridging ligand at the active site. Instead, an extra sulfur atom is observed binding Ni and Se, leading to a SeCys conformation that shields the NiFe site from contact with oxygen. This structure provides several insights that may explain the fast activation and O(2) tolerance of these enzymes. |
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Authors:
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Marta C Marques; Ricardo Coelho; Antonio L De Lacey; In?s A C Pereira; Pedro M Matias |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-21 |
Journal Detail:
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Title: Journal of molecular biology Volume: 396 ISSN: 1089-8638 ISO Abbreviation: J. Mol. Biol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-03-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985088R Medline TA: J Mol Biol Country: England |
Other Details:
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Languages: eng Pagination: 893-907 Citation Subset: IM |
Copyright Information:
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(c) 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Instituto de Tecnologia Qu?mica e Biol?gica, Universidade Nova de Lisboa, Apartado 127, 2781-901 Oeiras, Portugal. |
| Data Bank Information | |
Bank Name/Acc. No.:
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PDB/2WPN; R2WPNSF |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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chemistry*,
isolation & purification Catalytic Domain Crystallography, X-Ray Desulfovibrio vulgaris / enzymology* Hydrogenase / chemistry*, isolation & purification Hydrophobicity Ligands Models, Molecular Oxidation-Reduction Protein Conformation Spectroscopy, Fourier Transform Infrared Static Electricity |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Ligands; EC 1.12.-/nickel-iron-selenium hydrogenase; EC 1.12.7.2/Hydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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