Document Detail


Three-Dimensional Rotation of Mouse Embryos.
MedLine Citation:
PMID:  22231670     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Research and clinical applications, such as microinjection and polar body biopsy involve three-dimensional rotation of mammalian oocytes/embryos. In these cell manipulation tasks, the polar body of an embryo/oocyte must be made visible and properly oriented under optical microscopy. Cell rotation in conventional manual operation by skilled professionals is based on trial and error, such as through repeated vacuum aspiration and release. The randomness of this manual procedure, its poor reproducibility, and inconsistency across operators entail a systematic technique for automated, non-invasive, three-dimensional rotational control of single cells. This paper reports a system that tracks the polar body of mouse embryos in real time and controls multiple motion control devices to conduct automated threedimensional rotational control of mouse embryos. Experimental results demonstrated the system¡¯s capability for polar body orientation with a high success rate of 90%, an accuracy of 1.9¡ã, and an average speed of 22.8 sec/cell (vs. averagely 40 sec/cell in manual operation).
Authors:
C Leung; Z Lu; X Zhang; Y Sun
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-05
Journal Detail:
Title:  IEEE transactions on bio-medical engineering     Volume:  -     ISSN:  1558-2531     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0012737     Medline TA:  IEEE Trans Biomed Eng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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