Document Detail

Three-dimensional microstructural changes in murine abdominal aortic aneurysms quantified using immunofluorescent array tomography.
MedLine Citation:
PMID:  22140132     Owner:  NLM     Status:  MEDLINE    
This study investigated the spatial and temporal remodeling of blood vessel wall microarchitecture and cellular morphology during abdominal aortic aneurysm (AAA) development using immunofluorescent array tomography (IAT), a high-resolution three-dimensional (3D) microscopy technology, in the murine model. Infrarenal aortas of C57BL6 mice (N=20) were evaluated at 0, 7, and 28 days after elastase or heat-inactivated elastase perfusion. Custom algorithms quantified volume fractions (VF) of elastin, smooth muscle cell (SMC) actin, and adventitial collagen type I, as well as elastin thickness, elastin fragmentation, non-adventitial wall thickness, and nuclei amount. The 3D renderings depicted elastin and collagen type I degradation and SMC morphological changes. Elastin VF decreased 37.5% (p<0.01), thickness decreased 48.9%, and fragmentation increased 449.7% (p<0.001) over 28 days. SMC actin VF decreased 78.3% (p<0.001) from days 0 to 7 and increased 139.7% (p<0.05) from days 7 to 28. Non-adventitial wall thickness increased 61.1%, medial nuclei amount increased 159.1% (p<0.01), and adventitial collagen type I VF decreased 64.1% (p<0.001) over 28 days. IAT and custom image analysis algorithms have enabled robust quantification of vessel wall content, microstructure, and organization to help elucidate the dynamics of vascular remodeling during AAA development.
Sanaz Saatchi; Junya Azuma; Nishey Wanchoo; Stephen J Smith; Paul G Yock; Charles A Taylor; Philip S Tsao
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2011-12-01
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  60     ISSN:  1551-5044     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-25     Completed Date:  2012-03-30     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  97-109     Citation Subset:  IM    
Department of Bioengineering, Stanford University, Stanford, California 94305, USA.
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MeSH Terms
Aortic Aneurysm, Abdominal / pathology*
Fluorescent Antibody Technique / methods*
Imaging, Three-Dimensional / methods*
Mice, Inbred C57BL
Microscopy, Fluorescence / methods*
Tomography / methods*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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