Document Detail

Three-dimensional folding and functional organization principles of the Drosophila genome.
MedLine Citation:
PMID:  22265598     Owner:  NLM     Status:  MEDLINE    
Chromosomes are the physical realization of genetic information and thus form the basis for its readout and propagation. Here we present a high-resolution chromosomal contact map derived from a modified genome-wide chromosome conformation capture approach applied to Drosophila embryonic nuclei. The data show that the entire genome is linearly partitioned into well-demarcated physical domains that overlap extensively with active and repressive epigenetic marks. Chromosomal contacts are hierarchically organized between domains. Global modeling of contact density and clustering of domains show that inactive domains are condensed and confined to their chromosomal territories, whereas active domains reach out of the territory to form remote intra- and interchromosomal contacts. Moreover, we systematically identify specific long-range intrachromosomal contacts between Polycomb-repressed domains. Together, these observations allow for quantitative prediction of the Drosophila chromosomal contact map, laying the foundation for detailed studies of chromosome structure and function in a genetically tractable system.
Tom Sexton; Eitan Yaffe; Ephraim Kenigsberg; Frédéric Bantignies; Benjamin Leblanc; Michael Hoichman; Hugues Parrinello; Amos Tanay; Giacomo Cavalli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-19
Journal Detail:
Title:  Cell     Volume:  148     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-06     Completed Date:  2012-03-19     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  458-72     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Cell Nucleus / genetics
Chromosomes, Insect
Drosophila Proteins / metabolism
Drosophila melanogaster / cytology,  embryology,  genetics*
Genome, Insect*
Models, Statistical
Polycomb Repressive Complex 1
Grant Support
232947//European Research Council
Reg. No./Substance:
0/Drosophila Proteins; 0/Polycomb protein, Drosophila; EC Repressive Complex 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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