Document Detail


Three-Dimensional Anatomy of the Ciona intestinalis Tailbud Embryo at Single-cell Resolution.
MedLine Citation:
PMID:  23022659     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
During embryogenesis, chordates pass through a tailbud stage in which the larval tail is formed. Since acquisition of a tadpole-like tail during tailbud stage is one of the key events in the evolution of chordates, understanding the anatomy of the tailbud stage chordate embryo is of special interest. In this study, to understand comprehensively the anatomy of the tailbud embryo at single-cell-level, real microscopic image stacks of the tailbud embryo in Ciona intestinalis were reconstructed into a 3D computer model. This comprehensive 3D model of the ascidian tailbud embryo was based on real images of confocal laser scanning microscope (CLSM) and therefore, cell shape, location and cell arrangement reflect real geometries of the tailbud embryo. We found that the tailbud embryo consists of 1580 cells, including 836 epidermal cells, 228 cells in the central nervous system, 219 mesenchymal cells, four trunk ventral cells, two B/B⁎8.11 cells, 36 muscle cells, 40 notochord cells, four primordial germ cells, and 199 endodermal cells. Moreover, we identified for the first time two populations of previously undefined cells (a total of 12 cells) in Ciona: one located in the lateral trunk and the other located under the tail dorsal epidermis. This information provides a first step for understanding how the body plan of the chordate tailbud embryo formed and evolved.
Authors:
Mitsuru J Nakamura; Jun Terai; Reiko Okubo; Kohji Hotta; Kotaro Oka
Related Documents :
24020119 - Preparation and in vitro anti-tumor properties of toad venom extract-loaded solid lipid...
23799549 - The effect of mapk inhibitors and ros modulators on cell growth and death of h2o2‑tre...
24554029 - Low expression of erk signaling pathway affecting proliferation, cell cycle arrest and ...
24231369 - Quantification of healthy and atretic germ cells and follicles in the developing and po...
23335179 - Evidence for a crucial role of paneth cells in mediating the intestinal response to inj...
23868059 - H-ras transfers from b to t cells via tunneling nanotubes.
19021259 - Conversion of a phase- to a rate-coded position signal by a three-stage model of theta ...
21974829 - Long-term monitoring of live cell proliferation in presence of pvp-hypericin: a new str...
15936999 - In vitro toxicity test of ethyl 2-cyanoacrylate, a tissue adhesive used in cardiovascul...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  Developmental biology     Volume:  -     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A cis-element in the Notch1 locus is involved in the regulation of gene expression in interneuron pr...
Next Document:  An oocyte-specific astacin family protease, alveolin, is released from cortical granules to trigger ...