Document Detail

A three-component mechanism for fibroblast migration with a contractile cell body that couples a myosin II-independent propulsive anterior to a myosin II-dependent resistive tail.
MedLine Citation:
PMID:  22398722     Owner:  NLM     Status:  MEDLINE    
To understand the mechanism of cell migration, we cultured fibroblasts on micropatterned tracks to induce persistent migration with a highly elongated morphology and well-defined polarity, which allows microfluidic pharmacological manipulations of regional functions. The function of myosin II was probed by applying inhibitors either globally or locally. Of interest, although global inhibition of myosin II inhibited tail retraction and caused dramatic elongation of the posterior region, localized inhibition of the cell body inhibited nuclear translocation and caused elongation of the anterior region. In addition, local application of cytochalasin D at the tip inhibited frontal extension without inhibiting forward movement of the cell nucleus, whereas local treatment posterior to the nucleus caused reversal of nuclear movement. Imaging of cortical dynamics indicated that the region around the nucleus is a distinct compression zone where activities of anterior and posterior regions converge. These observations suggest a three-component model of cell migration in which a contractile middle section is responsible for the movement of a bulky cell body and the detachment/retraction of a resistive tail, thereby allowing these regions to undergo coordinated movement with a moving anterior region that carries little load.
Wei-hui Guo; Yu-li Wang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-07
Journal Detail:
Title:  Molecular biology of the cell     Volume:  23     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-30     Completed Date:  2012-11-23     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1657-63     Citation Subset:  IM    
Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15219, USA.
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MeSH Terms
Actins / metabolism*
Cell Movement / physiology*
Cell Surface Extensions
Cells, Cultured
Fibroblasts / cytology*,  metabolism*
Myosin Type II / metabolism*
NIH 3T3 Cells
Grant Support
Reg. No./Substance:
0/Actins; EC 3.6.1.-/Myosin Type II

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