| Third trimester amniotic fluid cells with the capacity to develop neural phenotypes and with heterogeneity among sub-populations. | |
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MedLine Citation:
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PMID: 22377907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Our aim was the search for new sources of cells potentially useful for central nervous system regenerative medicine. Extra-embryonic tissues are promising sources of pluripotent stem cells. Among these, human second-trimester amniotic fluid (AF) contains cell populations exhibiting self-renewal capacity, multipotency and the expression of embryonic cell markers. METHODS: Here we report the properties of the easily available third-trimester AF cells (AFCs). Different cell types from 6 of 9 AF samples were separated, expanded, and characterized by assessing their morphological, proliferative, and differentiative properties. RESULTS: All isolated cultures presented CD105, CD90 and CD73 mesenchymal markers, whereas they differed among themselves in CD117, CD146, CD31, NG2 and CD133 expression. Their doubling time and telomere length were conserved throughout many passages. Importantly, immunofluorescence and Real-time PCR showed that, during their proliferative state and differentiation, several cultures expressed neuronal and glial markers such as nestin, GFAP, β-tubulin III and neurofilament H indicating their potential attitude towards a neural fate. Indeed, these cells showed a rather poor capacity to differentiate in adipogenic and osteogenic lineages. CONCLUSIONS: In this work we report that cells with neural differentiation capability can be isolated from third-trimester AF, such properties could be useful for neuro-regenerative purposes. |
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Authors:
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Daniele Bottai; Daniela Cigognini; Emanuela Nicora; Monica Moro; Maria Grazia Grimoldi; Raffaella Adami; Sergio Abrignani; Anna Maria Marconi; Anna Maria Di Giulio; Alfredo Gorio |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Restorative neurology and neuroscience Volume: 30 ISSN: 1878-3627 ISO Abbreviation: Restor. Neurol. Neurosci. Publication Date: 2012 |
Date Detail:
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Created Date: 2012-03-01 Completed Date: 2012-06-25 Revised Date: 2013-04-10 |
Medline Journal Info:
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Nlm Unique ID: 9005499 Medline TA: Restor Neurol Neurosci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 55-68 Citation Subset: IM |
Affiliation:
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Department of Medicine, Surgery and Dentistry, Faculty of Medicine, University of Milan Via A. di Rudinì, Milan, Italy. daniele.bottai@unimi.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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cytology* Antigens / genetics, metabolism Antigens, CD / genetics, metabolism Cell Differentiation / genetics, physiology* Cell Proliferation Cells, Cultured Female Fetus / cytology, physiology Flow Cytometry Gene Expression Regulation, Developmental / physiology Glial Fibrillary Acidic Protein / genetics, metabolism Humans Intermediate Filament Proteins / genetics, metabolism Karyotyping / methods Nerve Tissue Proteins / genetics, metabolism Octamer Transcription Factor-3 / genetics, metabolism Phenotype Pluripotent Stem Cells / classification, physiology* Pregnancy Pregnancy Trimester, Third* Proteoglycans / genetics, metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/Antigens, CD; 0/Glial Fibrillary Acidic Protein; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Octamer Transcription Factor-3; 0/Proteoglycans; 0/chondroitin sulfate proteoglycan 4; 0/nestin |
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