Document Detail


Third trimester amniotic fluid cells with the capacity to develop neural phenotypes and with heterogeneity among sub-populations.
MedLine Citation:
PMID:  22377907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Our aim was the search for new sources of cells potentially useful for central nervous system regenerative medicine. Extra-embryonic tissues are promising sources of pluripotent stem cells. Among these, human second-trimester amniotic fluid (AF) contains cell populations exhibiting self-renewal capacity, multipotency and the expression of embryonic cell markers.
METHODS: Here we report the properties of the easily available third-trimester AF cells (AFCs). Different cell types from 6 of 9 AF samples were separated, expanded, and characterized by assessing their morphological, proliferative, and differentiative properties.
RESULTS: All isolated cultures presented CD105, CD90 and CD73 mesenchymal markers, whereas they differed among themselves in CD117, CD146, CD31, NG2 and CD133 expression. Their doubling time and telomere length were conserved throughout many passages. Importantly, immunofluorescence and Real-time PCR showed that, during their proliferative state and differentiation, several cultures expressed neuronal and glial markers such as nestin, GFAP, β-tubulin III and neurofilament H indicating their potential attitude towards a neural fate. Indeed, these cells showed a rather poor capacity to differentiate in adipogenic and osteogenic lineages.
CONCLUSIONS: In this work we report that cells with neural differentiation capability can be isolated from third-trimester AF, such properties could be useful for neuro-regenerative purposes.
Authors:
Daniele Bottai; Daniela Cigognini; Emanuela Nicora; Monica Moro; Maria Grazia Grimoldi; Raffaella Adami; Sergio Abrignani; Anna Maria Marconi; Anna Maria Di Giulio; Alfredo Gorio
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Restorative neurology and neuroscience     Volume:  30     ISSN:  1878-3627     ISO Abbreviation:  Restor. Neurol. Neurosci.     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-01     Completed Date:  2012-06-25     Revised Date:  2013-04-10    
Medline Journal Info:
Nlm Unique ID:  9005499     Medline TA:  Restor Neurol Neurosci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  55-68     Citation Subset:  IM    
Affiliation:
Department of Medicine, Surgery and Dentistry, Faculty of Medicine, University of Milan Via A. di Rudinì, Milan, Italy. daniele.bottai@unimi.it
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MeSH Terms
Descriptor/Qualifier:
Amniotic Fluid / cytology*
Antigens / genetics,  metabolism
Antigens, CD / genetics,  metabolism
Cell Differentiation / genetics,  physiology*
Cell Proliferation
Cells, Cultured
Female
Fetus / cytology,  physiology
Flow Cytometry
Gene Expression Regulation, Developmental / physiology
Glial Fibrillary Acidic Protein / genetics,  metabolism
Humans
Intermediate Filament Proteins / genetics,  metabolism
Karyotyping / methods
Nerve Tissue Proteins / genetics,  metabolism
Octamer Transcription Factor-3 / genetics,  metabolism
Phenotype
Pluripotent Stem Cells / classification,  physiology*
Pregnancy
Pregnancy Trimester, Third*
Proteoglycans / genetics,  metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Antigens; 0/Antigens, CD; 0/Glial Fibrillary Acidic Protein; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Octamer Transcription Factor-3; 0/Proteoglycans; 0/chondroitin sulfate proteoglycan 4; 0/nestin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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