Document Detail

Third ventricular coinjection of subthreshold doses of NPY and AgRP stimulate food hoarding and intake and neural activation.
MedLine Citation:
PMID:  22012701     Owner:  NLM     Status:  MEDLINE    
We previously demonstrated that 3rd ventricular (3V) neuropeptide Y (NPY) or agouti-related protein (AgRP) injection potently stimulates food foraging/hoarding/intake in Siberian hamsters. Because NPY and AgRP are highly colocalized in arcuate nucleus neurons in this and other species, we tested whether subthreshold doses of NPY and AgRP coinjected into the 3V stimulates food foraging, hoarding, and intake, and/or neural activation [c-Fos immunoreactivity (c-Fos-ir)] in hamsters housed in a foraging/hoarding apparatus. In the behavioral experiment, each hamster received four 3V treatments by using subthreshold doses of NPY and AgRP for all behaviors: 1) NPY, 2) AgRP, 3) NPY+AgRP, and 4) saline with a 7-day washout period between treatments. Food foraging, intake, and hoarding were measured 1, 2, 4, and 24 h and 2 and 3 days postinjection. Only when NPY and AgRP were coinjected was food intake and hoarding increased. After identical treatment in separate animals, c-Fos-ir was assessed at 90 min and 14 h postinjection, times when food intake (0-1 h) and hoarding (4-24 h) were uniquely stimulated. c-Fos-ir was increased in several hypothalamic nuclei previously shown to be involved in ingestive behaviors and the central nucleus of the amygdala (CeA), but only in NPY+AgRP-treated animals (90 min and 14 h: magno- and parvocellular regions of the hypothalamic paraventricular nucleus and perifornical area; 14 h only: CeA and sub-zona incerta). These results suggest that NPY and AgRP interact to stimulate food hoarding and intake at distinct times, perhaps released as a cocktail naturally with food deprivation to stimulate these behaviors.
Brett J W Teubner; Erin Keen-Rhinehart; Timothy J Bartness
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-10-19
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  302     ISSN:  1522-1490     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-26     Completed Date:  2012-03-26     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R37-48     Citation Subset:  IM    
Department of Biology, Georgia State University, Atlanta, 30302-4010, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Agouti-Related Protein / administration & dosage,  pharmacology*
Amygdala / metabolism
Dose-Response Relationship, Drug
Eating / drug effects*,  psychology
Feeding Behavior / drug effects*,  psychology
Hypothalamus / metabolism
Injections, Intraventricular
Models, Animal
Neurons / metabolism
Neuropeptide Y / administration & dosage,  pharmacology*
Obsessive Hoarding / psychology*
Proto-Oncogene Proteins c-fos / metabolism*
Grant Support
Reg. No./Substance:
0/Agouti-Related Protein; 0/Neuropeptide Y; 0/Proto-Oncogene Proteins c-fos

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Subcellular localization of skeletal muscle lipid droplets and PLIN family proteins OXPAT and ADRP a...
Next Document:  Rapid simultaneous determination of multiple pesticide residues in traditional Chinese medicines usi...