Document Detail


Thioredoxin and thioredoxin reductase show function-related changes in the gastric mucosa: immunohistochemical evidence.
MedLine Citation:
PMID:  3227877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Low levels of thioredoxin and thioredoxin reductase immunoreactivity were demonstrated immunohistochemically in rat gastric epithelial cells. The intensity was influenced by feeding and fasting, the former resulting in diminished reactions. Acute vagotomy, which abolishes basal acid secretion, resulted in a strongly increased thioredoxin immunoreactivity in all gastric epithelial cells. Stimulation of vagotomized rats with pentagastrin and carbachol reduced the levels of thioredoxin and thioredoxin reductase. Atropine and omeprazole (in stimulated, vagotomized rats) completely inhibited acid secretion, but caused different effects on the thioredoxin levels of gastric cells. Atropine restored the thioredoxin immunoreactivity in most gastric epithelial cells to that of the unstimulated, vagotomized controls. Omeprazole, however, did not reverse the effects of stimulation, and, except in the parietal cells, weaker fluorescence was observed. Similar reaction patterns were seen for thioredoxin reductase, although at lower staining intensities. The results demonstrate that thioredoxin and thioredoxin reductase are expressed in resting cells, and to a lower extent in cells with ongoing secretion.
Authors:
H A Hansson; H F Helander; A Holmgren; B Rozell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  132     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1989-03-27     Completed Date:  1989-03-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  313-20     Citation Subset:  IM    
Affiliation:
Department of Histology, University of Göteborg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Atropine / pharmacology
Bacterial Proteins / metabolism*
Carbachol / pharmacology
Gastric Acid / secretion
Gastric Mucosa / cytology,  metabolism*
Immunohistochemistry
Male
NADH, NADPH Oxidoreductases / metabolism*
Omeprazole / pharmacology
Pentagastrin / pharmacology
Rats
Rats, Inbred Strains
Thioredoxin-Disulfide Reductase / metabolism*
Thioredoxins / metabolism*
Vagotomy
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 51-55-8/Atropine; 51-83-2/Carbachol; 52500-60-4/Thioredoxins; 5534-95-2/Pentagastrin; 73590-58-6/Omeprazole; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.8.1.9/Thioredoxin-Disulfide Reductase

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