Document Detail


Thioredoxin-family protein EYE2 and Ser/Thr kinase EYE3 play interdependent roles in eyespot assembly.
MedLine Citation:
PMID:  21372178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The eyespot of the biflagellate unicellular green alga Chlamydomonas reinhardtii is a complex organelle that facilitates directional responses of the cell to environmental light stimuli. The eyespot, which assembles de novo after every cell division and is associated with the daughter four-membered (D4) microtubule rootlet, comprises an elliptical patch of rhodopsin photoreceptors on the plasma membrane and stacks of carotenoid-rich pigment granule arrays in the chloroplast. Two loci, EYE2 and EYE3, define factors involved in the formation and organization of the eyespot pigment granule arrays. Whereas EYE3, a serine/threonine kinase of the ABC1 family, localizes to pigment granules, EYE2 localization corresponds to an area of the chloroplast envelope in the eyespot. EYE2 is positioned along, and adjacent to, the D4 rootlet in the absence of pigment granules. The eyespot pigment granule array is required for maintenance of the elliptical shape of both the overlying EYE2 and channelrhodopsin-1 photoreceptor patches. We propose a model of eyespot assembly wherein rootlet and photoreceptor direct EYE2 to an area of the chloroplast envelope, where it acts to facilitate assembly of pigment granule arrays, and EYE3 plays a role in the biogenesis of the pigment granules.
Authors:
Joseph S Boyd; Telsa M Mittelmeier; Mary Rose Lamb; Carol L Dieckmann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-03-03
Journal Detail:
Title:  Molecular biology of the cell     Volume:  22     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-02     Completed Date:  2011-11-09     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1421-9     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Cell Membrane
Chlamydomonas reinhardtii / genetics,  metabolism*
Chloroplasts
Immunoblotting
Membrane Proteins
Organoids
Photoreceptors, Plant / metabolism*
Pigments, Biological*
Plant Proteins / metabolism*
Protein Multimerization*
Protein-Serine-Threonine Kinases / metabolism*
Sequence Alignment
Sequence Analysis, DNA
Signal Transduction
Grant Support
ID/Acronym/Agency:
T32GM008659/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Photoreceptors, Plant; 0/Pigments, Biological; 0/Plant Proteins; 0/eye2 protein, Chlamydomonas reinhardtii; EC 2.7.11.1/Protein-Serine-Threonine Kinases
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