| Thioredoxin deficiency in yeast prolongs S phase and shortens the G1 interval of the cell cycle. | |
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MedLine Citation:
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PMID: 2026619 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two thioredoxin genes from the yeast Saccharomyces cerevisiae were cloned using synthetic oligonucleotide probes. The DNA sequences of the two genes were found to be 74% identical. The two genes, designated TRX1 and TRX2, were mutagenized in vitro and used to construct a set of thioredoxin deletion mutants. The loss of either thioredoxin gene alone has no effect on cell growth or morphology. However, the simultaneous deletion of both thioredoxin genes profoundly affects the cell cycle. S phase is 3-fold longer, and G1 is virtually absent. In addition, the thioredoxin double mutant shows a 33% increase in generation time, a significant increase in cell size, and a greater proportion of large budded cells. The results suggest that in the absence of TRX1 and TRX2, a slow rate of DNA replication inhibits the normal progress of cellular reproduction. Surprisingly, the loss of both thioredoxins also leads to methionine auxotrophy. Thus yeast glutaredoxin is unable to substitute for thioredoxin in sulfate assimilation. As a first step in studying the cell cycle control mechanisms that respond to the thioredoxin deficiency, it was shown that cell viability does not require the function of RAD9, a known cell cycle checkpoint. |
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Authors:
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E G Muller |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 266 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1991 May |
Date Detail:
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Created Date: 1991-06-10 Completed Date: 1991-06-10 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9194-202 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Washington, Seattle, 98195. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M57623; M57624; M60203; M60204; M60205; M60206; M62647; M62648; M62780; M63262 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cell Cycle* Chromosome Deletion Cloning, Molecular Genes, Fungal* Molecular Sequence Data Oligonucleotides / chemistry Restriction Mapping S Phase Saccharomyces cerevisiae / cytology, genetics* Thioredoxins / genetics*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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GM45448/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides; 52500-60-4/Thioredoxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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