| Thioflavin T fluorescence assay for beta-lactoglobulin fibrils hindered by DAPH. | |
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MedLine Citation:
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PMID: 19063973 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The molecule 4,5-dianilinophthalimide was recently found to be an efficient compound in disaggregating amyloid fibrils involved in the Alzheimer's disease. In this study we have investigated whether the compound 4,5-dianilinophthalimide was able to disaggregate fibrils derived from beta-lactoglobulin. In addition to a Thioflavin T fluorescence assay, flow-induced birefringence was used as an independent technique to measure the total length concentration of the fibrils. An additional advantage of the latter technique is that not only the total length concentration, but also the length distribution of the fibrils can be measured. The results from flow-induced birefringence showed that the total amount of fibrils and also the length distribution of the fibrils was not influenced by the addition of 4,5-dianilinophthalimide, even though this was suggested by the results of the Thioflavin T assay. The results of flow-induced birefringence were confirmed by rheological measurements and transmission electron microscopy. Our findings show that the use of a Thioflavin T assay in order to probe the possible disaggregating effect of certain compounds can give misleading results. |
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Authors:
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Ardy Kroes-Nijboer; Yvette S Lubbersen; Paul Venema; Erik van der Linden |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-03 |
Journal Detail:
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Title: Journal of structural biology Volume: 165 ISSN: 1095-8657 ISO Abbreviation: J. Struct. Biol. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-02-04 Completed Date: 2009-04-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9011206 Medline TA: J Struct Biol Country: United States |
Other Details:
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Languages: eng Pagination: 140-5 Citation Subset: IM |
Affiliation:
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Laboratory of Physics and Physical Chemistry of Foods, Department of Agrotechnology and Food Sciences, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amyloid
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chemistry* Birefringence Circular Dichroism Fluorescent Dyes / analysis, chemistry Lactoglobulins / chemistry* Microscopy, Electron, Transmission Phthalimides / chemistry* Rheology Spectrometry, Fluorescence Thiazoles / chemistry* Viscosity |
| Chemical | |
Reg. No./Substance:
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0/Amyloid; 0/Fluorescent Dyes; 0/Lactoglobulins; 0/Phthalimides; 0/Thiazoles; 157168-02-0/4,5-dianilinophthalimide; 2390-54-7/thioflavin T |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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