Document Detail

Thin-layer chromatographic separation of sulfated and nonsulfated lithocholic acids and their glycine and taurine conjugates.
MedLine Citation:
PMID:  1127355     Owner:  NLM     Status:  MEDLINE    
A method for superior thin-layer chromatographic separation of lithocholic acid and its N-glycine and N-taurine conjugates, as well as their respective 3alpha-sulfates, is described. A solvent system of chloroform-methanol-acetic acid-water 65:24:15:9 (v/v) is used with air-dried plates of silicic acid containing calcium sulfate (10% by weight) under conditions of chamber saturation.
O W Cass; A E Cowen; A F Hofmann; S B Coffin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  16     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1975 Mar 
Date Detail:
Created Date:  1975-07-20     Completed Date:  1975-07-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  159-60     Citation Subset:  IM    
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MeSH Terms
Cholic Acids / isolation & purification*
Chromatography, Thin Layer
Evaluation Studies as Topic
Glycine / isolation & purification*
Lithocholic Acid / isolation & purification*
Sulfuric Acids / isolation & purification
Taurine / isolation & purification*
Reg. No./Substance:
0/Cholic Acids; 0/Sulfuric Acids; 107-35-7/Taurine; 434-13-9/Lithocholic Acid; 56-40-6/Glycine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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