Document Detail


Thin layer chromatographic analysis of glucose and maltose in estivated Biomphalaria glabrata snails and those infected with Schistosoma mansoni.
MedLine Citation:
PMID:  17010650     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thin layer chromatography was used to analyze the glucose and maltose concentrations of the digestive gland-gonad complex (DGG) of uninfected-estivated Biomphalaria glabrata snails and estivated B. glabrata patently infected with Schistosoma mansoni. All snails were estivated in a most chamber at a relative humidity of 98+/-1% and a temperature of 23+/-1 degrees C for 14 days. Carbohydrates were extracted from the DGG with 70% aqueous ethanol, and extracts were analyzed on silica gel preadsorbent plates using ethyl acetate-glacial acetic acid-methanol-water (60:15:15:10) mobile phase, alpha-naphthol-sulfuric acid detection reagent, and quantification by densitometry. The concentrations of glucose and maltose were significantly reduced in both uninfected-estivated snails and infected-estivated snails.
Authors:
Jamie A Jarusiewicz; Joseph Sherma; Bernard Fried
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-09-03
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  145     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:    2006 Nov-Dec
Date Detail:
Created Date:  2006-12-12     Completed Date:  2007-02-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  346-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Lafayette College, Easton, PA 18042, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomphalaria / chemistry,  parasitology*,  physiology*
Chromatography, Thin Layer / methods
Diet
Digestive System / chemistry
Estivation*
Glucose / analysis*,  metabolism
Host-Parasite Interactions
Maltose / analysis*,  metabolism
Schistosoma mansoni / growth & development*
Sensitivity and Specificity
Grant Support
ID/Acronym/Agency:
N01-AI-55270/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
50-99-7/Glucose; 69-79-4/Maltose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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