| Thin layer chromatographic analysis of glucose and maltose in estivated Biomphalaria glabrata snails and those infected with Schistosoma mansoni. | |
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MedLine Citation:
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PMID: 17010650 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thin layer chromatography was used to analyze the glucose and maltose concentrations of the digestive gland-gonad complex (DGG) of uninfected-estivated Biomphalaria glabrata snails and estivated B. glabrata patently infected with Schistosoma mansoni. All snails were estivated in a most chamber at a relative humidity of 98+/-1% and a temperature of 23+/-1 degrees C for 14 days. Carbohydrates were extracted from the DGG with 70% aqueous ethanol, and extracts were analyzed on silica gel preadsorbent plates using ethyl acetate-glacial acetic acid-methanol-water (60:15:15:10) mobile phase, alpha-naphthol-sulfuric acid detection reagent, and quantification by densitometry. The concentrations of glucose and maltose were significantly reduced in both uninfected-estivated snails and infected-estivated snails. |
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Authors:
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Jamie A Jarusiewicz; Joseph Sherma; Bernard Fried |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-09-03 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology Volume: 145 ISSN: 1096-4959 ISO Abbreviation: Comp. Biochem. Physiol. B, Biochem. Mol. Biol. Publication Date: 2006 Nov-Dec |
Date Detail:
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Created Date: 2006-12-12 Completed Date: 2007-02-06 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9516061 Medline TA: Comp Biochem Physiol B Biochem Mol Biol Country: England |
Other Details:
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Languages: eng Pagination: 346-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Lafayette College, Easton, PA 18042, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomphalaria / chemistry, parasitology*, physiology* Chromatography, Thin Layer / methods Diet Digestive System / chemistry Estivation* Glucose / analysis*, metabolism Host-Parasite Interactions Maltose / analysis*, metabolism Schistosoma mansoni / growth & development* Sensitivity and Specificity |
| Grant Support | |
ID/Acronym/Agency:
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N01-AI-55270/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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50-99-7/Glucose; 69-79-4/Maltose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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