Document Detail

Thin-film tunable filters for hyperspectral fluorescence microscopy.
MedLine Citation:
PMID:  24077519     Owner:  NLM     Status:  MEDLINE    
Hyperspectral imaging is a powerful tool that acquires data from many spectral bands, forming a contiguous spectrum. Hyperspectral imaging was originally developed for remote sensing applications; however, hyperspectral techniques have since been applied to biological fluorescence imaging applications, such as fluorescence microscopy and small animal fluorescence imaging. The spectral filtering method largely determines the sensitivity and specificity of any hyperspectral imaging system. There are several types of spectral filtering hardware available for microscopy systems, most commonly acousto-optic tunable filters (AOTFs) and liquid crystal tunable filters (LCTFs). These filtering technologies have advantages and disadvantages. Here, we present a novel tunable filter for hyperspectral imaging-the thin-film tunable filter (TFTF). The TFTF presents several advantages over AOTFs and LCTFs, most notably, a high percentage transmission and a high out-of-band optical density (OD). We present a comparison of a TFTF-based hyperspectral microscopy system and a commercially available AOTF-based system. We have characterized the light transmission, wavelength calibration, and OD of both systems, and have then evaluated the capability of each system for discriminating between green fluorescent protein and highly autofluorescent lung tissue. Our results suggest that TFTFs are an alternative approach for hyperspectral filtering that offers improved transmission and out-of-band blocking. These characteristics make TFTFs well suited for other biomedical imaging devices, such as ophthalmoscopes or endoscopes.
Peter Favreau; Clarissa Hernandez; Ashley Stringfellow Lindsey; Diego F Alvarez; Thomas Rich; Prashant Prabhat; Silas J Leavesley
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  19     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2013-09-30     Completed Date:  2014-01-13     Revised Date:  2014-09-28    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  011017     Citation Subset:  IM    
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MeSH Terms
Endothelial Cells / chemistry,  cytology
Green Fluorescent Proteins / chemistry
Image Processing, Computer-Assisted / methods*
Microscopy, Fluorescence / instrumentation*,  methods*
Optical Imaging
Grant Support
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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