Document Detail


Thin filament-reconstituted skinned muscle fibers for the study of muscle physiology.
MedLine Citation:
PMID:  22131807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We review the use of thin filament-reconstituted muscle fibers in the study of muscle physiology. Thin filament extraction and reconstitution protocol is a powerful technique to study the role of each component of the thin filament. It is also useful for studying the properties of genetically modified molecules such as actin and tropomyosin. We also review the combination of this protocol with sinusoidal analysis, which will provide a solid technique for determining the effect of regulatory proteins on actomyosin interaction and concomitant cross-bridge kinetics. We suggest that thin filament-reconstituted muscle fibers are an ideal system for studying muscle physiology especially when gene modifications of actin or tropomyosin are involved.
Authors:
Sayaka Higuchi; Yoshikazu Tsukasaki; Norio Fukuda; Satoshi Kurihara; Hideaki Fujita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2011-11-03
Journal Detail:
Title:  Journal of biomedicine & biotechnology     Volume:  2011     ISSN:  1110-7251     ISO Abbreviation:  J. Biomed. Biotechnol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-12-01     Completed Date:  2012-03-22     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101135740     Medline TA:  J Biomed Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  486021     Citation Subset:  IM    
Affiliation:
Laboratory for Comprehensive Bioimaging, Riken Quantitative Biology Center, OLABB, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / metabolism,  physiology*
Actins / genetics,  metabolism
Actomyosin / genetics,  metabolism
Calcium / physiology
Cytoskeleton / physiology*
Humans
Muscle Fibers, Skeletal / metabolism,  physiology*
Muscle Proteins / genetics,  metabolism
Muscle, Striated / physiology*
Protein Kinases / genetics,  metabolism
Sarcomeres / metabolism,  physiology
Tropomyosin / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Actins; 0/Muscle Proteins; 0/Tropomyosin; 0/connectin; 7440-70-2/Calcium; 9013-26-7/Actomyosin; EC 2.7.-/Protein Kinases
Comments/Corrections

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