Document Detail

Thin filament-reconstituted skinned muscle fibers for the study of muscle physiology.
MedLine Citation:
PMID:  22131807     Owner:  NLM     Status:  MEDLINE    
We review the use of thin filament-reconstituted muscle fibers in the study of muscle physiology. Thin filament extraction and reconstitution protocol is a powerful technique to study the role of each component of the thin filament. It is also useful for studying the properties of genetically modified molecules such as actin and tropomyosin. We also review the combination of this protocol with sinusoidal analysis, which will provide a solid technique for determining the effect of regulatory proteins on actomyosin interaction and concomitant cross-bridge kinetics. We suggest that thin filament-reconstituted muscle fibers are an ideal system for studying muscle physiology especially when gene modifications of actin or tropomyosin are involved.
Sayaka Higuchi; Yoshikazu Tsukasaki; Norio Fukuda; Satoshi Kurihara; Hideaki Fujita
Related Documents :
4013987 - Therapy of digoxin intoxication in dogs by specific hemoperfusion through agarose polya...
22127247 - Thymosins and muscle regeneration.
17326937 - Bartonella quintana endocarditis in dogs.
25155217 - Forelimb and hindlimb ground reaction forces of walking cats: assessment and comparison...
9320497 - Glycerol synthesis in the rainbow smelt osmerus mordax
23325407 - Airway epithelium-derived relaxing factor: myth, reality or naivety?
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2011-11-03
Journal Detail:
Title:  Journal of biomedicine & biotechnology     Volume:  2011     ISSN:  1110-7251     ISO Abbreviation:  J. Biomed. Biotechnol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-12-01     Completed Date:  2012-03-22     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101135740     Medline TA:  J Biomed Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  486021     Citation Subset:  IM    
Laboratory for Comprehensive Bioimaging, Riken Quantitative Biology Center, OLABB, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Actin Cytoskeleton / metabolism,  physiology*
Actins / genetics,  metabolism
Actomyosin / genetics,  metabolism
Calcium / physiology
Cytoskeleton / physiology*
Muscle Fibers, Skeletal / metabolism,  physiology*
Muscle Proteins / genetics,  metabolism
Muscle, Striated / physiology*
Protein Kinases / genetics,  metabolism
Sarcomeres / metabolism,  physiology
Tropomyosin / genetics,  metabolism
Reg. No./Substance:
0/Actins; 0/Muscle Proteins; 0/Tropomyosin; 0/connectin; 7440-70-2/Calcium; 9013-26-7/Actomyosin; EC 2.7.-/Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Emerging functions of transcription factors in malaria parasite.
Next Document:  Immunodiagnosis of neurocysticercosis: ways to focus on the challenge.