Document Detail


Thiazolidinediones exhibit different effects on preadipocytes isolated from rat mesenteric fat tissue and cell line 3T3-L1 cells derived from mice.
MedLine Citation:
PMID:  17350863     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of PPAR-gamma agonists, thiazolidinediones (TZDs), on preadipocytes isolated from rat mesenteric adipose tissue and murine cell line 3T3-L1 were compared using an in vitro cell culture system. After each cell formed a confluent monolayer under appropriate medial conditions, pioglitazone or troglitazone was applied at 10 microM to each medium for cell maturation. We observed morphological changes in each cell, especially the accumulation of lipid droplets in the cytoplasm, during the culture periods. At the end of culture, DNA content, triglyceride (TG) content and glycerol-3-phosphate dehydrogenase (GPDH) activity were determined. Adiponectin concentrations in each culture medium were also measured during appropriate experimental periods. Application of TZDs increased the DNA content, TG accumulation and GPDH activity in the 3T3-L1 cells but not in the mesenteric adipocytes. Although TG accumulation was unchanged, the number of lipid particles was decreased and the size of lipid particles in the mesenteric adipocytes was increased by TZD application. Although the TZDs increased adiponectin release from the 3T3-L1 cells, adiponectin release from mesenteric adipocytes was suppressed (P<0.05). Thus, the effects of TZDs differed between the primary culture of mesenteric adipose cells and the line cell culture of 3T3-L1 cells. The source of adipocytes is an important factor in determining the action of TZDs in vitro, and particular attention should be paid when evaluating the effect of PPAR-gamma agonists on adipose tissues.
Authors:
Hitoshi Mineo; Chikako Oda; Hideyuki Chiji; Teruo Kawada; Kyoko Shimizu; Toshio Taira
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2007-01-14
Journal Detail:
Title:  Cell biology international     Volume:  31     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-05-25     Completed Date:  2007-08-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  703-10     Citation Subset:  IM    
Affiliation:
Primary Cell Co., Ltd., Center for Advanced Science and Technology 306, Hokkaido University, Kita-21 Nishi-11, Sapporo, Hokkaido 001-0021, Japan. mineo@primarycell.com
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MeSH Terms
Descriptor/Qualifier:
3T3-L1 Cells
Adipocytes / chemistry,  drug effects*,  metabolism
Adiponectin / metabolism
Animals
Cell Proliferation / drug effects
DNA / analysis,  drug effects
Glycerolphosphate Dehydrogenase / metabolism
Intra-Abdominal Fat / cytology,  drug effects*
Male
Mesentery / cytology
Mice
Rats
Rats, Sprague-Dawley
Thiazolidinediones / pharmacology*
Triglycerides / analysis
Chemical
Reg. No./Substance:
0/Adiponectin; 0/Thiazolidinediones; 0/Triglycerides; 9007-49-2/DNA; EC 1.1.-/Glycerolphosphate Dehydrogenase

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