Document Detail

Thiamine pyrophosphate as an effector of 2-oxoglutarate dehydrogenase complex from European bison heart.
MedLine Citation:
PMID:  8653071     Owner:  NLM     Status:  MEDLINE    
The purified 2-oxoglutarate dehydrogenase complex (OGDC) from the European bison heart was near saturated with endogenous bound thiamine pyrophosphate (TPP). Exogenous TPP added to the full OGDC reaction medium decreased S0.5 for 2-oxoglutarate approximately 2.6-fold without any notable change in the maximum reaction rate. The TPP effect was observed in the presence of 1 mM ADP which alone is a strong positive allosteric effector of OGDC. At an unsaturating 2-oxoglutarate concentration the A50 value for TPP was approximately 0.05 mM. The ADP-like action of exogenous TPP was also found in the 2-oxoglutarate dehydrogenase (E1) reaction, determined in the presence of 2,6-dichlorophenoloindophenol as an electron acceptor.
S Strumilo; J Markiewicz
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry and molecular biology international     Volume:  37     ISSN:  1039-9712     ISO Abbreviation:  Biochem. Mol. Biol. Int.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1996-07-31     Completed Date:  1996-07-31     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9306673     Medline TA:  Biochem Mol Biol Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  101-6     Citation Subset:  IM    
Department of Biochemistry, University of Warsaw, Bialystok, Poland.
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MeSH Terms
Adenosine Diphosphate / metabolism,  pharmacology
Calcium / metabolism,  pharmacology
Dose-Response Relationship, Drug
Ketoglutarate Dehydrogenase Complex / drug effects*,  metabolism*
Myocardium / enzymology*
Thiamine Pyrophosphate / metabolism*,  pharmacology*
Reg. No./Substance:
154-87-0/Thiamine Pyrophosphate; 58-64-0/Adenosine Diphosphate; 7440-70-2/Calcium; EC Dehydrogenase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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