Document Detail


Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes.
MedLine Citation:
PMID:  15528987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies have demonstrated that thiamine (vitamin B1) has a cytoprotective effect against ischemic damage to the heart, and that heat shock protein 70 (Hsp70) is capable of protecting cardiac cells from lethal ischemia/hypoxia. We show here that thiamine has a cytoprotective effect on cultured neonatal rat cardiomyocytes under hypoxic insult, and also protects the cardiomyocytes against hypoxia-induced apoptosis; caspase-3 activation, PARP cleavage and DNA fragmentation are all inhibited. Moreover, it increases the level of Hsp70 protein in the cardiomyocytes even under prolonged hypoxic stress and its effects on hypoxia-induced cardiac cell death are antagonized by an Hsp70 inhibitor. These results suggest that the cytoprotective effect of thiamine in cardiomyocytes under hypoxic stress is due to its ability to induce Hsp70.
Authors:
Bo Hee Shin; Seung Hyuk Choi; Eun Young Cho; Min-Jeong Shin; Ki-Chul Hwang; Hong Keun Cho; Ji Hyung Chung; Yangsoo Jang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecules and cells     Volume:  18     ISSN:  1016-8478     ISO Abbreviation:  Mol. Cells     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-11-05     Completed Date:  2005-04-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  133-40     Citation Subset:  IM    
Affiliation:
Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Anoxia / pathology*
Apoptosis / drug effects*
Caspase 3
Caspases / metabolism
Cells, Cultured
DNA Fragmentation
HSP70 Heat-Shock Proteins / physiology
Myocytes, Cardiac / drug effects
Poly(ADP-ribose) Polymerases / metabolism
Protective Agents / pharmacology
Rats
Rats, Sprague-Dawley
Thiamine / pharmacology*
Chemical
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/Protective Agents; 59-43-8/Thiamine; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

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