Document Detail


Thermostatic animal platform for intravital microscopy of thin tissues.
MedLine Citation:
PMID:  14609527     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a novel temperature-controlled, all-on-board design platform for intravital microscopy of thin tissues in small laboratory animals. The apparatus uses transparent heaters and miniature controllers to control independently the temperature of the tissue pedestal and animal heating pad, as well as the animal core temperature. The system ensures a uniform temperature for a thin tissue placed on the surface of a transparent window and maintenance of the animal's core temperature without overheating. This platform provides an alternative to warm superfusion solution for in vivo microscopy, under circumstances where a well-controlled temperature is required. All components of the apparatus are commercially available, inexpensive and reliable, thereby simplifying its assembly. The platform is convenient for use with trans- and epi-illumination and does not interfere with movement of the microscope stage.
Authors:
Aleksander S Golub; Roland N Pittman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Microvascular research     Volume:  66     ISSN:  0026-2862     ISO Abbreviation:  Microvasc. Res.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-11     Completed Date:  2004-07-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0165035     Medline TA:  Microvasc Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  213-7     Citation Subset:  IM    
Affiliation:
Department of Physiology, P.O. Box 980551, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298-0551, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Temperature
Hot Temperature
Microscopy / instrumentation*,  methods*
Muscles / pathology
Physiology / instrumentation*
Rats
Temperature
Time Factors
Grant Support
ID/Acronym/Agency:
HL18292/HL/NHLBI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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