Document Detail


Thermoreversible copolymer gels for extracellular matrix.
MedLine Citation:
PMID:  10813747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To improve the properties of a reversible synthetic extracellular matrix based on a thermally reversible polymer, copolymers of N-isopropylacrylamide and acrylic acid were prepared in benzene with varying contents of acrylic acid (0 to 3%) and the thermal properties were evaluated. The poly(N-isopropylacrylamide) and copolymers made with acrylic acid had molecular weights from 0.8 to 1.7 x10(6) D. Differential scanning calorimetry (DSC) showed the high-molecular-weight acrylic acid copolymers had similar onset temperatures to the homopolymers, but the peak width was considerably increased with increasing acrylic acid content. DSC and cloud point measurements showed that polymers with 0 to 3% acrylic acid exhibit a lower critical solution temperature (LCST) transition between 30 degrees and 37 degrees C. In swelling studies, the homopolymer showed significant syneresis at temperatures above 31 degrees C. Copolymers with 1 and 1.5% showed syneresis beginning at 32 degrees and 37 degrees C, respectively. At 37 degrees C the copolymers with 1.5-3% acrylic acid showed little or no syneresis. Due to the high water content and a transition near physiologic conditions (below 37 degrees C), the polymers with 1.5-2.0% acrylic acid exhibited properties that would be useful in the development of a refillable synthetic extracellular matrix. Such a matrix could be applied to several cell types, including islets of Langerhans, for a biohybrid artificial pancreas.
Authors:
B Vernon; S W Kim; Y H Bae
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biomedical materials research     Volume:  51     ISSN:  0021-9304     ISO Abbreviation:  J. Biomed. Mater. Res.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-09-25     Completed Date:  2000-09-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0112726     Medline TA:  J Biomed Mater Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  69-79     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Inc.
Affiliation:
Department of Bioengineering, University of Utah, Salt Lake City, Utah 84112, USA.
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MeSH Terms
Descriptor/Qualifier:
Acrylamides* / chemical synthesis
Acrylates* / analysis,  chemical synthesis
Calorimetry, Differential Scanning
Extracellular Matrix*
Gels
Hot Temperature
Molecular Weight
Pancreas, Artificial
Polymers
Solutions
Temperature
Water / analysis
Grant Support
ID/Acronym/Agency:
DK46458/DK/NIDDK NIH HHS; GM08393/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Acrylamides; 0/Acrylates; 0/Gels; 0/Polymers; 0/Solutions; 2210-25-5/N-isopropylacrylamide; 7732-18-5/Water; 79-10-7/acrylic acid

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