| Thermoprecipitation of lysozyme from egg white using copolymers of N-isopropylacrylamide and acidic monomers. | |
| | |
MedLine Citation:
|
PMID: 11278034 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Thermoprecipitation of lysozyme from egg white was demonstrated using copolymers of N-isopropylacrylamide with acrylic acid, methacrylic acid, 2-acryloylamido-2-methylpropane-sulfonic acid and itaconic acid, respectively. Polymers synthesized using molar feed ratio of N-isopropylacrylamide:acidic monomers of 98:2 exhibited lower critical solution temperatures in the range of 33--35 degrees C. These polymers exhibited electrostatic interactions with lysozyme and inhibited its bacteriolytic activity. The concentration of acidic groups required to attain 50% relative inhibition of lysozyme by the polymers, was 10(4)--10(5) times lower than that required for the corresponding monomers. This was attributed to the multimeric nature of polymer-lysozyme binding. More than 90% lysozyme activity was recovered from egg white. Polymers exhibited reusability up to at least 16 cycles with retention of >85% recovery of specific activity from aqueous solution. In contrast, copolymer comprising natural inhibitor of lysozyme i.e. poly (N-isopropylacrylamide-co-O-acryloyl N-acetylglucosamine) lost 50% recovery of specific activity. Thermoprecipitation using these copolymers, which enables very high recovery of lysozyme from egg white, would be advantageous over pH sensitive polymers, which generally exhibit lower recovery. |
| | |
Authors:
|
A A Vaidya; B S Lele; M G Kulkarni; R A Mashelkar |
Related Documents
:
|
17245744 - Novel cholic acid functionalized star oligo/poly(dl-lactide)s for biomedical applications. 993224 - Poly(glycolic acid) sutures in canine vascular anastomoses. 12711514 - Protein adsorption, fibroblast activity and antibacterial properties of poly(3-hydroxyb... 19053294 - Gold nanoparticles protected with thiol-derivatized amphiphilic poly(epsilon-caprolacto... 1376144 - An unusual coloured compound in digests of senile nuclear cataract lens proteins. 6811604 - Role of stimulation of arachidonic acid release in the proliferative response of 3t3 mo... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of biotechnology Volume: 87 ISSN: 0168-1656 ISO Abbreviation: J. Biotechnol. Publication Date: 2001 May |
Date Detail:
|
Created Date: 2001-03-30 Completed Date: 2001-08-09 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8411927 Medline TA: J Biotechnol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 95-107 Citation Subset: IM |
Affiliation:
|
Chemical Engineering Division, National Chemical Laboratory, Polymer Science and Engineering Group, Pune 411 008, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylglucosamine
/
chemistry Acrylamides / chemistry* Acrylates / chemistry* Animals Binding Sites Chemical Precipitation Egg White* Hydrogen-Ion Concentration Ions Methacrylates / chemistry Muramidase / antagonists & inhibitors, isolation & purification*, metabolism Osmolar Concentration Polymers / chemistry* Solutions Succinates / chemistry |
| Chemical | |
Reg. No./Substance:
|
0/Acrylamides; 0/Acrylates; 0/Ions; 0/Methacrylates; 0/N-isopropylacrylamide-acrylic acid copolymer; 0/Polymers; 0/Solutions; 0/Succinates; 2210-25-5/N-isopropylacrylamide; 7512-17-6/Acetylglucosamine; 79-10-7/acrylic acid; 79-41-4/methacrylic acid; 97-65-4/itaconic acid; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A concept of welfare based on reward evaluating mechanisms in the brain: anticipatory behaviour as a...
Next Document: Lipase catalyzed synthesis of organic acid esters of lactic acid in non-aqueous media.