Document Detail

Thermophoretic Manipulation of DNA Translocation through Nanopores.
MedLine Citation:
PMID:  23199225     Owner:  NLM     Status:  Publisher    
Manipulating DNA translocation through nanopore is one crucial requirement for new ultra-fast sequencing methods in the sense that the polymers have to be denatured, unraveled and then propelled through the pore with very low speed. Here we propose and theoretically explore a novel design to fulfill the demands by utilizing cross-pore thermal gradient. The high temperature in cis reservoir is expected to transform double-stranded DNA into single strands, and that temperature would also prevent those single strands from intra-strand base-pairing, thus achieving favorable polymer conformation for the subsequent translocation and sequencing. Then, the substantial temperature drop across the pore caused by the thermal-insulating membrane separating cis and trans chambers would stimulate thermophoresis of the molecules through nanopores. Our theoretical evaluation shows that the DNA translocation speeds will be orders smaller than the electrophoretic counterpart, while high capture rate of DNA into nanopore is maintained, both of which would greatly benefit the sequencing.
Yuhui He; Makusu Tsutsui; Ralph H Scheicher; Fan Bai; Masateru Taniguchi; Tomoji Kawai
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-2
Journal Detail:
Title:  ACS nano     Volume:  -     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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